[交流] 【求助/交流】求教做免疫共沉淀的人

我沒做過免疫共沉淀，現(xiàn)在要設(shè)計免疫共沉淀實驗，為了更高的成功率和實驗質(zhì)量，現(xiàn)在請做過的高手簡要介紹一下免疫共沉淀最可能遇到的幾種問題

回復(fù)此樓

» 猜你喜歡

不合理蛙科研實驗中的趣事：實驗器材的 “烏龍” 已經(jīng)有0人回復(fù)
不合理蛙科研實驗中的趣事：和實驗材料的 “斗智斗勇” 已經(jīng)有0人回復(fù)
化學(xué)工程及工業(yè)化學(xué)論文潤色/翻譯怎么收費? 已經(jīng)有284人回復(fù)
蛋白質(zhì)檢測：精準(zhǔn)分析，解鎖生物分子的密碼已經(jīng)有0人回復(fù)
不合理蛙科研實驗之小鼠實驗：嚴(yán)謹(jǐn)設(shè)計，解析生命機制的重要載體已經(jīng)有0人回復(fù)
不合理蛙科研實驗之重金屬檢測：精準(zhǔn)篩查，守護健康與環(huán)境的防線已經(jīng)有0人回復(fù)
不合理蛙科研實驗之“蛙測重金屬我背鍋三千” 已經(jīng)有0人回復(fù)
不合理蛙科研實驗之“鼠逃三次我發(fā)三篇SCI” 已經(jīng)有0人回復(fù)
納米粒度分析已經(jīng)有3人回復(fù)
林科院林化所招收材料與化工專業(yè)碩士，坐標(biāo)南京已經(jīng)有0人回復(fù)

» 本主題相關(guān)價值貼推薦，對您同樣有幫助:

GST pull down 實驗細(xì)節(jié)問題求教已經(jīng)有5人回復(fù)
1X PBS 的意思已經(jīng)有7人回復(fù)
想請教一下做植物蛋白的免疫共沉淀需要特別注意什么嗎？已經(jīng)有6人回復(fù)
關(guān)于做染色質(zhì)免疫共沉淀的幾個問題，請高手賜教已經(jīng)有7人回復(fù)
請教相互作用能的問題已經(jīng)有7人回復(fù)
求助quantity one 已經(jīng)有10人回復(fù)
免疫共沉淀和染色質(zhì)免疫共沉淀后如何驗證已經(jīng)有7人回復(fù)
可以做一個沉淀分子的MD嗎？比如氫氧化鋅沉淀已經(jīng)有4人回復(fù)
求教共沉淀問題已經(jīng)有6人回復(fù)
請教一個免疫共沉淀技術(shù)的問題已經(jīng)有4人回復(fù)
SDS-PAGE求教�。。。。。�！已經(jīng)有8人回復(fù)
蛋白質(zhì)相互作用？已經(jīng)有4人回復(fù)
為何配的SDS electrophoresis running buffer總有沉淀？已經(jīng)有10人回復(fù)
【求助】共沉淀求助已經(jīng)有8人回復(fù)
【求助/交流】求助免疫共沉淀實驗已經(jīng)有20人回復(fù)
【交流】尋找做免疫磁珠的同仁~交流探討已經(jīng)有17人回復(fù)
【求助/交流】關(guān)于免疫共沉淀的問題已經(jīng)有3人回復(fù)
【求助/交流】求助~~~關(guān)于免疫共沉淀~~~ 已經(jīng)有8人回復(fù)
【求助/交流】染色質(zhì)免疫共沉淀已經(jīng)有18人回復(fù)
【求助/交流】轉(zhuǎn)錄調(diào)控蛋白的研究方法已經(jīng)有4人回復(fù)
【求助/交流】求助免疫球蛋白純化后有沉淀的事情！已經(jīng)有3人回復(fù)
【求助】關(guān)于免疫反應(yīng)問題求教。謝謝！已經(jīng)有4人回復(fù)

» 搶金幣啦！回帖就可以得到:

查看全部散金貼

1樓 2010-12-10 16:37:12

已閱關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

xhjggl

木蟲 (正式寫手)

★ ★
dhd997(金幣+2):熱心 2010-12-11 08:42:16
jurkat.1640(金幣+3):謝謝參與 2010-12-11 08:58:14

上海生工有試劑盒賣，很好用！
Immunoprecipitation Kit
Components BS688 For 20 reactions BS689 For 20 reactions BS690 For 20 reactions
10x Lysis buffer 10 ml 10 ml 10 ml
PMSF 0.6ml 0.6ml 0.6ml
10x IP 50 ml 50 ml 50 ml
SDS 5 ml 5 ml 5 ml
NaCl 10 ml 10 ml 10 ml
Protein A-Agarose 0.4 ml / /
Protein A/G Plus-Agarose / 0.4 ml /
Protein A-Sepharose / / 0.6 ml
20x PBS 20 ml 20 ml 20 ml
1x SDS gel-loading buffer 1 ml 1ml 1 ml
Spin columns and tubes 20 20 20
Price ¥1,520 ¥2,250 ¥1,920
Description:
Immunoprecipitation (IP) is a method by which a protein can be specifically purified from a complex mixture of proteins using a specific antibody and a matrix that binds the antibody. The matrix bound protein (via the specific antibody) can then be separated from the mixture by centrifugation. The matrices commonly used are agarose or sepharose bound Protein A or protein A/G PlUS. IP is one of the most widely used immunochemical techniques. IP followed by SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and immunoblotting is routinely used in a variety of applications: to measure the molecular weights of protein antigens, study protein/protein interactions, determine specific enzymatic activity, monitor protein post-translational modifications and determine the presence and quantity of proteins. The IP technique also enables the detection of rare proteins, which otherwise would be difficult to detect, since immunoprecipitation can concentrate them as much as 10,000-fold..
Applications:The Protein-A Immunoprecipitation Kit is especially designed to allow maximal recovery of immunoprecipitates. The whole process is performed in mini-spin columns, instead of in microcentrifuge tubes, which enables convenient washing of the antigen-antibody-bound beads.
Features:Easy access.;Minimal loss of antigen-antibody bound beads during washing.；Minimal non-specific signals by increasing the stringency of the washing steps.
Storage Condition:Stored at 4℃.and PMSF should be stored at -20℃.

贊一下(1人)

回復(fù)此樓

2樓2010-12-10 18:51:57

已閱關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

mourning

木蟲 (正式寫手)

應(yīng)助: 1 (幼兒園)
金幣: 1972.7
帖子: 582
在線: 88.6小時
蟲號: 426626

jurkat.1640(金幣+2):謝謝 2010-12-11 08:58:58

問題就是可能有非特異性曝出來

贊一下

回復(fù)此樓

3樓2010-12-10 23:11:23

已閱關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

jurkat.1640

至尊木蟲 (文壇精英)

MolEPI: 1
應(yīng)助: 908 (博后)
金幣: 17150.7
帖子: 14518
在線: 2183.8小時
蟲號: 514340

引用回帖:

Originally posted by mourning at 2010-12-10 23:11:23:
問題就是可能有非特異性曝出來

蛋白質(zhì)的上樣量大概多少？

贊一下

回復(fù)此樓

4樓2010-12-14 16:04:51

已閱關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

vetzhangshen

鐵蟲 (初入文壇)

應(yīng)助: 0 (幼兒園)
金幣: 18.5
帖子: 42
在線: 12.2小時
蟲號: 1616086

★
小木蟲(金幣+0.5):給個紅包，謝謝回帖

啊怎么沒有人回復(fù)啦

贊一下

回復(fù)此樓

5樓2012-02-22 10:54:29

已閱關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

最具人氣熱帖推薦 [查看全部]	作者	回/看	最后發(fā)表

[考研] 材料，紡織，生物（0856、0710），化學(xué)招生啦 +3	Eember. 2026-03-17	9/450	2026-03-18 10:28 by Eember.
[基金申請] 被我言中：新模板不強調(diào)格式了，假專家開始管格式了 +4	beefly 2026-03-14	4/200	2026-03-17 22:04 by 黃鳥于飛Chao
[考研] 085601材料工程專碩求調(diào)劑 +5	慕寒mio 2026-03-16	5/250	2026-03-17 21:31 by hmn_wj
[考研] 332求調(diào)劑 +6	Zz版 2026-03-13	6/300	2026-03-17 17:03 by ruiyingmiao
[考研] 085600材料與化工 +4	安全上岸！ 2026-03-16	4/200	2026-03-17 14:02 by 勇敢太監(jiān)王公公
[考研] 0854可跨調(diào)劑，一作一項核心論文五項專利，省、國級證書40+數(shù)一英一287 +3	小李0854 2026-03-16	3/150	2026-03-17 13:40 by 熱情沙漠
[考研] 機械專碩325，尋找調(diào)劑院校 +3	y9999 2026-03-15	5/250	2026-03-16 19:58 by y9999
[考研] 304求調(diào)劑 +4	ahbd 2026-03-14	4/200	2026-03-16 16:48 by 我的船我的海
[考研] 283求調(diào)劑 +10	小樓。 2026-03-12	14/700	2026-03-16 16:08 by 13811244083
[考研] 326求調(diào)劑 +3	mlpqaz03 2026-03-15	3/150	2026-03-16 07:33 by Iveryant
[考研] 求老師收留調(diào)劑 +4	jiang姜66 2026-03-14	5/250	2026-03-15 20:11 by Winj1e
[考研] 080500，材料學(xué)碩302分求調(diào)劑學(xué)校 +4	初識可樂 2026-03-14	5/250	2026-03-14 21:08 by peike
[考研] 265求調(diào)劑 +4	威化餅07 2026-03-12	4/200	2026-03-14 17:23 by userper
[考研] 0856材料與化工301求調(diào)劑 +5	奕束光 2026-03-13	5/250	2026-03-13 22:00 by 星空星月
[考研] 304求調(diào)劑 +7	7712b 2026-03-13	7/350	2026-03-13 21:42 by peike
[考研] （081700）化學(xué)工程與技術(shù)-298分求調(diào)劑 +12	11啦啦啦 2026-03-11	35/1750	2026-03-13 21:25 by JourneyLucky
[考研] 考研調(diào)劑 +4	芬達46 2026-03-12	4/200	2026-03-13 16:04 by ruiyingmiao
[考研] 材料專碩350 求調(diào)劑 +4	王金科 2026-03-12	4/200	2026-03-13 16:02 by ruiyingmiao
[考研] 一志愿山大07化學(xué) 332分四六級已過本科山東雙非求調(diào)劑！ +3	不想理你 2026-03-12	3/150	2026-03-13 14:18 by JourneyLucky
[考研] 0817化學(xué)工程與技術(shù)考研312分調(diào)劑 +3	T123 tt 2026-03-12	3/150	2026-03-13 10:49 by houyaoxu