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| 采用細(xì)胞病變抑制法檢測(cè)重組蛋白在雞胚成纖維細(xì)胞上抗新城疫病毒復(fù)制的活性,結(jié)果表明純化的雞的IFN-α和IFN-γ具有一定的抗新城疫病毒復(fù)制的活性.將兩個(gè)蛋白按照不同比例混合檢測(cè)抗新城疫病毒復(fù)制的活性,結(jié)果顯示將豬IFN-α和IFN-γ重組蛋白按照125:1的比例進(jìn)行混合具有較強(qiáng)的活性. |
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| By CPE inhibition test to detect the duplicated activity of recombinant protein to resist the Newcastle Disease Virus on Chicken Embryo Fibroblast,the results showed that the IFN-α and IFN-γ of chicken’s had some duplicated activities to risist the Newcastle Disease Virus,Mixed two proteins at different proportions to detect the duplicated activity of resist to Newcastle Disease Virus,the results revealed that mixing the IFN-α and IFN-γ of pigs at the proportions of 125:1 will have strong activity. |
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The activity of anti-replication of Newcastle disease virus(NDV) of recombinant protein on Chicken Embryo Fibroblast was measured by cytopathic effect inhibition(CPEI).the results indicated that the IFN-α and IFN-γ of chicken had some activity of anti-replication of NDV. Mixed two proteins at different proportions to detect activity of anti-replication of NDV,the results showed that mixing the recombination proteins IFN-α and IFN-γ of pig(樓主這里到底是豬還是雞) according to the ratio of 125:1,the mixture would have stronger activity of anti-replication of NDV. |
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