版塊導(dǎo)航: 正在加載中...

登錄注冊

應(yīng)《網(wǎng)絡(luò)安全法》要求，自2017年10月1日起，未進(jìn)行實(shí)名認(rèn)證將不得使用互聯(lián)網(wǎng)跟帖服務(wù)。為保障您的帳號能夠正常使用，請盡快對帳號進(jìn)行手機(jī)號驗(yàn)證，感謝您的理解與支持！

24小時熱門版塊排行榜

返回列表

langlang0715

銅蟲 (著名寫手)

應(yīng)助: 1 (幼兒園)
金幣: 431.8
帖子: 1322
在線: 233.6小時
蟲號: 498207

[交流] 【求助/交流】2° antibody表示什么意思

看文獻(xiàn)有個2° antibody是什么意思？是存放溫度嗎。在下不是學(xué)生物的，請生物方面的大俠們講詳細(xì)些呢
原文：
GBP Binding Assay. Printed slides were analyzed without any
further modification of the surface. Slides were incubated in
either a one-step procedure with labeled proteins or a sandwich
procedure in which the bound GBP was overlaid with labeled
secondary antibodies or GBPs precomplexed with labeled antibodies.
GBPs were added at a concentration of 5–50 gml in
buffer (usually PBS containing 0.005–0.5% Tween-20). Secondary
antibodies (10 gml in PBS) were overlaid on bound GBP.
GBP-antibody precomplexes were prepared in a molar ratio of
1:0.5:0.25 (5–50 gml) for GBP2° antibody3° antibody, respectively
(15 min on ice). The samples (50–100 l) were applied
either directly onto the surface of a single slide and covered with
a microscope coverslip or applied between two parallel slides
separated by thin tape and pressed together by paper clips (34)
and then incubated in a humidified chamber for 30–60 min.
Slides were subsequently washed by successive rinses in (i)
PBS-0.05% Tween, (ii) PBS, and (iii) deionized water, then
immediately subjected to imaging. Serum samples were typically
used at dilutions of 1:25, and 0.4–0.8 ml was applied directly onto
the slide surface without any cover glass. The slides were gently
rocked at room temperature for 90 min followed by detection
with secondary antibodies (Table 1). Whole virus was applied
(0.8 ml) at a concentration of 100 gml in buffer (PBS
containing 0.05% Tween-20) containing the neuraminidase inhibitor
oseltamivir carboxylate (10 M).

[ Last edited by langlang0715 on 2011-1-7 at 14:19 ]

回復(fù)此樓

» 猜你喜歡

298求調(diào)劑已經(jīng)有7人回復(fù)
263求調(diào)劑已經(jīng)有5人回復(fù)
276求調(diào)劑。有半年電池和半年高分子實(shí)習(xí)經(jīng)歷已經(jīng)有6人回復(fù)
求調(diào)劑材料學(xué)碩080500，總分289分已經(jīng)有21人回復(fù)
北科281學(xué)碩材料求調(diào)劑已經(jīng)有8人回復(fù)
2026化學(xué)調(diào)劑已經(jīng)有3人回復(fù)
08工學(xué)調(diào)劑已經(jīng)有9人回復(fù)
291 求調(diào)劑已經(jīng)有4人回復(fù)
070300化學(xué)求調(diào)劑已經(jīng)有5人回復(fù)
085600材料與化工已經(jīng)有10人回復(fù)

» 搶金幣啦！回帖就可以得到:

查看全部散金貼

1樓 2011-01-07 11:33:16

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

天龍n部

金蟲 (小有名氣)

應(yīng)助: 1 (幼兒園)
金幣: 403.7
帖子: 106
在線: 23.4小時
蟲號: 1118927

langlang0715(金幣+1):謝謝關(guān)注O(∩_∩)O~ 2011-01-07 14:08:06

抗體

回復(fù)此樓

2樓2011-01-07 11:44:57

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

lxj341401

至尊木蟲 (著名寫手)

antibody是抗體。

回復(fù)此樓

3樓2011-01-07 13:44:44

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

langlang0715

銅蟲 (著名寫手)

應(yīng)助: 1 (幼兒園)
金幣: 431.8
帖子: 1322
在線: 233.6小時
蟲號: 498207

引用回帖:

Originally posted by 天龍n部 at 2011-01-07 11:44:57:
抗體

那2°是什么意思呢？

贊一下

回復(fù)此樓

4樓2011-01-07 13:45:37

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

langlang0715

銅蟲 (著名寫手)

應(yīng)助: 1 (幼兒園)
金幣: 431.8
帖子: 1322
在線: 233.6小時
蟲號: 498207

引用回帖:

Originally posted by lxj341401 at 2011-01-07 13:44:44:
antibody是抗體。

2°抗體是什么意思呢？

贊一下

回復(fù)此樓

5樓2011-01-07 13:46:18

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

lxj341401

至尊木蟲 (著名寫手)

langlang0715(金幣+1):謝謝關(guān)注O(∩_∩)O~ 2011-01-07 14:07:40

引用回帖:

Originally posted by langlang0715 at 2011-01-07 13:46:18:

2°抗體是什么意思呢？

對抗體不熟悉，沒見過這樣的寫法。

贊一下

回復(fù)此樓

6樓2011-01-07 14:00:41

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

biolinkphila

新蟲 (小有名氣)

應(yīng)助: 0 (幼兒園)
金幣: 516.6
帖子: 100
在線: 37.6小時
蟲號: 1131296

提供原文就會知道

贊一下

回復(fù)此樓

7樓2011-01-07 14:13:00

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

路人甲007

木蟲 (正式寫手)

應(yīng)助: 18 (小學(xué)生)
金幣: 3797.9
帖子: 597
在線: 206.8小時
蟲號: 840731

建議把原文放上來起碼那句話得給吧

贊一下

回復(fù)此樓

8樓2011-01-07 14:14:39

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

dianaofyezi

金蟲 (正式寫手)

應(yīng)助: 1 (幼兒園)
金幣: 1660.1
帖子: 721
在線: 67.1小時
蟲號: 371239

langlang0715(金幣+5):非常感謝 2011-01-08 20:10:07

一般表示第二抗體，很多人簡寫都這么寫。

贊一下

回復(fù)此樓

9樓2011-01-08 19:46:34

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

相關(guān)版塊跳轉(zhuǎn) 我要訂閱樓主 langlang0715 的主題更新

返回列表

普通表情龍兔虎貓高級回復(fù) (可上傳附件)

最具人氣熱帖推薦 [查看全部]		作者	回/看	最后發(fā)表

[考研] 284求調(diào)劑 +6	Zhao anqi 2026-03-22	6/300	2026-03-23 09:23 by king123！
[考研] 315分，誠求調(diào)劑，材料與化工085600 +3	13756423260 2026-03-22	3/150	2026-03-22 20:11 by edmund7
[考研] 293求調(diào)劑 +12	zjl的號 2026-03-16	17/850	2026-03-22 16:51 by i_cooler
[考研] 305分求調(diào)劑（食品工程） +4	Sxy112 2026-03-21	6/300	2026-03-22 15:26 by 無懈可擊111
[考研] 能源材料化學(xué)課題組招收碩士研究生8-10名 +5	脫穎而出 2026-03-16	17/850	2026-03-22 15:18 by 脫穎而出
[考研] 生物學(xué)調(diào)劑 +5	Surekei 2026-03-21	5/250	2026-03-22 14:39 by tcx007
[考研] 考研調(diào)劑 +4	來好運(yùn)來來來 2026-03-21	4/200	2026-03-22 12:15 by 星空星月
[考博] 招收博士1-2人 +3	QGZDSYS 2026-03-18	4/200	2026-03-22 10:25 by QGZDSYS
[考研] 346求調(diào)劑[0856] +4	WayneLim327 2026-03-16	7/350	2026-03-21 04:02 by JourneyLucky
[考研] 070300化學(xué)319求調(diào)劑 +7	錦鯉0909 2026-03-17	7/350	2026-03-21 03:46 by JourneyLucky
[考研] 085700資源與環(huán)境308求調(diào)劑 +12	墨墨漠 2026-03-18	13/650	2026-03-21 01:42 by JourneyLucky
[考研] 一志愿武理材料305分求調(diào)劑 +6	想上岸的鯉魚 2026-03-18	7/350	2026-03-21 01:03 by JourneyLucky
[考研] 296求調(diào)劑 +6	www_q 2026-03-18	10/500	2026-03-20 23:56 by JourneyLucky
[考研] 材料專碩英一數(shù)二306 +7	z1z2z3879 2026-03-18	7/350	2026-03-20 23:48 by JourneyLucky
[考研] 一志愿武漢理工材料工程專碩調(diào)劑 +9	Doleres 2026-03-19	9/450	2026-03-20 22:36 by JourneyLucky
[考研] 生物學(xué)調(diào)劑招人�。�！ +3	山海天嵐 2026-03-17	4/200	2026-03-19 21:34 by 怎么釋懷
[考研] 【同濟(jì)軟件】軟件（085405）考研求調(diào)劑 +3	2026eternal 2026-03-18	3/150	2026-03-18 19:09 by 搏擊518
[考研] 考研求調(diào)劑 +3	橘頌. 2026-03-17	4/200	2026-03-17 21:43 by 有只貍奴
[考研] 一志愿蘇州大學(xué)材料工程（085601）專碩有科研經(jīng)歷三項(xiàng)國獎兩個實(shí)用型專利一項(xiàng)省級立項(xiàng) +6	大火山小火山 2026-03-16	8/400	2026-03-17 15:05 by 無懈可擊111
[考研] [導(dǎo)師推薦]西南科技大學(xué)國防/材料導(dǎo)師推薦 +3	尖角小荷 2026-03-16	6/300	2026-03-16 23:21 by 尖角小荷

24小時熱門版塊排行榜

[交流] 【求助/交流】2° antibody表示什么意思

» 猜你喜歡

» 搶金幣啦！回帖就可以得到: 查看全部散金貼

» 搶金幣啦！回帖就可以得到:

查看全部散金貼