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| 由于小球藻繁殖中營養(yǎng)物質的逐漸耗竭,有害代謝產物的逐漸積累,小球藻不可能始終保持高速度的無限繁殖。經(jīng)過一段時間后,小球藻繁殖速度漸減,死亡藻增多,藻增長率隨之下降并趨于停滯。穩(wěn)定期后藻繁殖越來越慢,死亡數(shù)越來越多,并超過活藻數(shù)量。該期藻形態(tài)顯著改變,出現(xiàn)衰退型或菌體自溶,生理代謝活動也趨于停滯。因此, 第一批藻種處理后的廢水達不到預期目標,需要接種新的小球藻種,進行二級培養(yǎng)。蛋白核小球藻在廢水中生長一段時間后進入穩(wěn)定期和衰亡期,此時藻細胞對廢水營養(yǎng)物質的需求量較少,廢水COD、蛋白質含量、鹽含量去除率比較低。用離心機離心分離后的廢水液COD在60~980之間,達不到排放標準。鑒于廢水中仍存有少量營養(yǎng)物質,本研究采用二次培養(yǎng)法將未經(jīng)馴化的藻種接種到一級廢水中繼續(xù)通氣培養(yǎng)。 |
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| During Chlorella reproduction, due to the gradual depletion of nutrients and the gradual accumulation of harmful metabolites, a high rate, unlimited reproduction can not be maintained. After a certain period of time, reproduction rate of Chlorella starts decreasing when dead Chlorella increases, resulting in reduced growth rate, which tends to stagnate. After the stationary phase, Chlorella reproduction rate becomes slower and slower. The increasing death finally outnumbers the live ones. Remarkable changes in the form of Chlorella take place, which include emergence of recession-type or cell autolysis, and stagnation of physiological, metabolic activity. Therefore, treating waste water by the first batch of Chlorella fails to achieve set objectives, but a second generation of Chlorella cultivation is required. After a certain period of time in the wastewater, Chlorella pyrenoidosa grows into the stationary phase and decline phase, when algal cells demand little nutrients from the wastewater, resulting in a low removal rate of COD, protein content and salt content. After centrifugal separation, COD still locates somewhere between 60 and 980, which does not meet emission standards. Given there is still a small amount of nutrients in wastewater, this study investigates a second generation cultivation of unaltered algae species in the first-level wastewater by aeration. |
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