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| 配制瓊脂濃度為 2 %的營養(yǎng)型固體培養(yǎng)基 ,滅菌后待溫度降至50 ℃左右時 ,在無菌條件下 ,將培養(yǎng)基倒入已滅菌并編號的培養(yǎng)皿( d ×h = 90 mm×15 mm)的皿蓋中 ,其厚度為皿蓋高度的 1/ 4左右為宜. 待培養(yǎng)基平板(為夾層的下層)冷卻后 ,將富集液分別按 10 - 2、 10 - 3、 10 - 4稀釋度吸取0. 2 mL 均勻涂布在平板上.靜置 ,待涂布液跡基本滲入培養(yǎng)基后 ,倒入同種營養(yǎng)型固體培養(yǎng)基(為夾層的上層) ,其厚度要求略薄于下層(約2~3 mm) ;倒上層時 ,讓液狀培養(yǎng)基形成凸起狀 ,隨即迅速將培養(yǎng)皿的內(nèi)皿(無菌)底朝下、口與皿蓋同向地嵌入上層培養(yǎng)基;結(jié)果是內(nèi)皿與培養(yǎng)基間無任何氣泡 ,內(nèi)皿周圍有少量培養(yǎng)基逸進內(nèi)外皿的側(cè)壁間隙內(nèi) ,最終表現(xiàn)出二重皿法樣的形式. |
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| The following is the instruction of how to prepare the 2% agar nutrient culture medium。Pour the agar liquid to the pre-ordered and sterile petri dish covers with the height about 1/ 4 of the cover’s height when it cools down to about 50 ℃ When the plate(the bottom layer of the sandwich) totally cools down to the room temperature,evenly coat 0. 2 mL富集液(專有名詞自己解決,以免誤導)on the plate with 10 - 2、 10 - 3、 10 – 4 titrated concentrations respectively. When the 富集液 largely penetrate into the plate, pour the same type of culture medium(the top layer of the sandwich) onto the plate in a convex shape with the thickness less than 2~3 mm of that of the plate. Then immediately cover the petri dish cover with the sterile 內(nèi)皿 and then a tiny fraction of the liquid medium culture will overflow into the sidewalls of the內(nèi)皿.Be careful not allow any air bubble form between the culture medium and the內(nèi)皿 and all of the above procedures are carried out on sterile condictions.Finally you’’ get 二重皿法樣 culture medium |



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