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北京石油化工學(xué)院2026年研究生招生接收調(diào)劑公告

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aswman08

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根據(jù)國家食品藥品監(jiān)督管理局《中藥注射劑指紋圖譜研究技術(shù)指南》、《中藥注射劑色譜指紋圖譜實(shí)驗(yàn)技術(shù)指南》和相關(guān)文獻(xiàn)，通過指紋圖譜方法學(xué)考查實(shí)驗(yàn)，確定了西洋參葉高效液相指紋圖譜的最佳色譜條件：色譜柱：十八烷基硅烷鍵合硅膠為填充劑；流動相：A：乙腈， B：水（0.05%磷酸，2%四氫呋喃）。柱溫為40℃，進(jìn)樣量為20μL，流速為1.0ml/min，檢測波長為203nm；0~18min，A：20%； 18~58min，A：20~40%（線性梯度）； 58~73min，A：40~100%（線性梯度）； 73~80min，100%。試驗(yàn)分析表明：西洋參葉指紋圖譜共有14個共有峰，產(chǎn)地為東北（長白、撫松、臨江、靖宇和朝陽）之間有優(yōu)良好的相似性（大于0.9），產(chǎn)地為山東（萊陽和文登）之間相似性較好，而東北和山東兩者之間的相似度略差（0.8～0.9），因此建議在選用藥材時，應(yīng)盡量選用地域接近的藥材，它們之間的相似度較好。

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1樓 2011-01-20 14:14:17

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aswman08(金幣+10): 2011-01-26 08:44:39

According to instructive rules of "Fingerprints of TCM Injections Technical Guide" and "Chromatographic Fingerprint of TCM injections Experimental Technology Guide " from the State Food and Drug Administration and related literatures, we set up the fingerprint chromatogram condition for the analysis of the leaves of Panax Quinquefolium Linn.The analysis was performed on octadecyl silane bonded silica column (column temperature:40 ℃) with acetonitrile (A) -0.05% phosphoric acid(B, contains 2% tetrahydrofuran) as mobile phase with gradient elution at flow rate of 1.0 mL/min. The UV detection wavelength was 203 nm. The gradient elution was carried out as follows: 0-18 min, A: 20%; 18-58 min, A: 20% to 40% (linear gradient); 58-73 min, A: 40% to 100% (linear gradient); 73 -80min, A: 100%. It was indicated that all the fingerprint chromatograms of the leaves of Panax Quinquefolium Linn had 14 shared peaks and that similarity of chromatograms of leaves of Panax Quinquefolium Linn varied from cultivation areas: chromtogram similarities of those from the Northeast were superior( >0.9); those from Shangdong were also good; but chromtogram similarity between thsoe from the Northeast and those from Shangdong was not so good (0.8-0.9). Thus, it is suggested that leaves of Panax Quinquefolium Linn from same cultivation area should be selected when needed because of better similarity.

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3樓2011-01-22 09:47:16

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糊涂豆

木蟲 (職業(yè)作家)

aswman08(金幣+5, 翻譯EPI+1): 2011-01-26 08:44:27

According to the State Food and Drug Administration"Fingerprints of TCM injections Technical Guide","chromatographic fingerprint of traditional Chinese medicine injections Experimental Technology Guide " and the relevant literature, we determined the best chromatographic conditions of the HPLC fingerprint of American ginseng leaves by fingerprint analysis experiment .
Column: octadecyl silane bonded silica as a filler;
Mobile phase: A: acetonitrile, B: water (0.05% phosphoric acid, 2% tetrahydrofuran).
Column temperature:40 ℃
Injection volume: 20μL,
Flow rate :1.0ml/min
Detection wavelength: 203nm; 0 ~ 18min A: 20%; 18 ~ 58min, A: 20 ~ 40% (linear gradient); 58 ~ 73min, A: 40 ~ 100% (linear gradient); 73 ~ 80min, 100%.

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2樓2011-01-22 08:14:06

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樓主？怎么不見回應(yīng)啊？

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4樓2011-01-24 08:50:22

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【懸賞金幣】回答本帖問題，作者aswman08將贈送你 15 個金幣
不短的文字啊，譯了樓主無動于衷讓人受不了

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5樓2011-01-24 08:53:23

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