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【求助/交流】誰幫解釋一下multimeric plasmid具體是什么含義
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| 看文章中有multimeric plasmid這個詞,沒有查到具體的定義,誰能詳細解釋一下這個詞的含義嗎?多謝! |
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木蟲之王 (文壇精英)
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多體質(zhì)粒,指不同的質(zhì)粒形式的混合物:超螺旋,開環(huán)。 http://www.qiagen.com/plasmid/agarosegelanalysis.aspx 上有說明 Lane 2: Multimeric forms of supercoiled plasmid DNA (pTZ19) which may be observed with some host strains, and should not be mistaken for genomic DNA. Multimeric plasmid DNA can easily be distinguished from genomic DNA by a simple restriction digestion: linearization of a plasmid sample displaying multimeric bands will yield a single defined band with the size of the linearized plasmid monomer (see lane 3). Lane 3: Linearized form of plasmid pTZ19 after restriction digestion with EcoRI. Lane 4: Sample contaminated with bacterial chromosomal DNA, which may be observed if the lysate is treated too vigorously (e.g., vortexing during incubation steps with Buffer P2 or P3). Genomic DNA contamination can easily be identified by digestion of the sample with EcoRI. A smear is observed, in contrast to the linear band seen after digestion of multimeric plasmid forms. |
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