 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 5 | 1/1 | 返回列表 |
| 查看: 1646 | 回復(fù): 13 | |||
| 本帖產(chǎn)生 1 個(gè) 翻譯EPI ，點(diǎn)擊這里進(jìn)行查看 | |||
| 當(dāng)前只顯示滿足指定條件的回帖，點(diǎn)擊這里查看本話題的所有回帖 | |||
[交流]
翻譯通順點(diǎn)
|
|||
| Extracts for ACC synthase (ACS) activity assays were prepared according to the method of Boller et al. (1979), and determined by the method of Lizada and Yang (1979) with slight modifications. One g tomato pericarp tissue was homogenized using a cold pestle and mortar in 5mL of extraction buffer which consisting of 4 mM dithiothreitol, 10mM pyridoxal phosphate and 1.0mM EDTA in 100mM pH 8.0 Hepes buffer. After centrifugation of the homogenate at 10,000×g for 20min, the supernatant was used for enzyme assays. All steps in the enzyme extraction were performed at 4◦C. ACC synthase activity was assayed in a 0.8mL reaction mixture containing 0.25mM S-adenosylmethionine, 50mM Hepes buffer and 10mM pyridoxal phosphate with 0.2mL of enzyme extract in test tubes. After incubation of the reaction mixture at 30 ◦C for 1 h, the tubes were sealed with a stopper and rubber septum, 0.1mL HgCl2 (80mM) and 0.1mL 5% NaOH–NaClO solution (1:2, v/v) were injected into the tubes, shaken and incubated for 2min. One mL of the gaseous portion was removed and assayed for ethylene as described above. ACS activity was expressed as nmol g−1 FWh−1. |
回復(fù)此樓» 猜你喜歡
291求調(diào)劑
已經(jīng)有7人回復(fù)
-
材料與化工272求調(diào)劑
已經(jīng)有13人回復(fù)
-
283求調(diào)劑（080500）
已經(jīng)有5人回復(fù)
-
材料科學(xué)與工程求調(diào)劑
已經(jīng)有5人回復(fù)
-
一志愿北京化工大學(xué)材料與化工（085600）296求調(diào)劑
已經(jīng)有17人回復(fù)
-
考研調(diào)劑
已經(jīng)有7人回復(fù)
-
343求調(diào)劑085601
已經(jīng)有3人回復(fù)
-
08工科求調(diào)劑286
已經(jīng)有3人回復(fù)
-
337求調(diào)劑
已經(jīng)有3人回復(fù)
-
329求調(diào)劑，一志愿西北工業(yè)大學(xué)，材料工程（085601）
已經(jīng)有10人回復(fù)
» 搶金幣啦！回帖就可以得到:
-
數(shù)一英一碩士調(diào)劑（雙一流高校）
+1/182
-
中國科學(xué)院青島生物能源與過程研究所 招生2-3名聯(lián)培生
+1/179
-
福建師范大學(xué)招收2026年化學(xué)、材料碩士3-4名
+1/90
-
五邑大學(xué)池振國教授團(tuán)隊(duì)2026年大量接收化學(xué)化工材料背景考研生
+5/85
-
湖北師范大學(xué)調(diào)劑 化學(xué)材料類
+1/78
-
五邑大學(xué)環(huán)境與化學(xué)工程學(xué)院先進(jìn)有機(jī)高分子材料團(tuán)隊(duì)
+1/42
-
博士科研人員（化學(xué)過程工藝與硬件系統(tǒng)工程）
+1/12
-
招收2026專業(yè)代碼08開頭學(xué)術(shù)型碩士研究生
+1/11
-
中國地震局地質(zhì)研究所+地球物理專業(yè)+接收調(diào)劑
+1/10
-
煙臺(tái)大學(xué)先進(jìn)陶瓷方向 招收材料專業(yè)調(diào)劑研究生
+1/10
-
三峽大學(xué)材料與化工學(xué)院調(diào)劑
+1/8
-
長江師范學(xué)院電子信息碩士和學(xué)科物理碩士調(diào)劑招生
+1/8
-
寧夏大學(xué)化學(xué)化工學(xué)院魏逸彬研究員課題組招聘啟事
+1/7
-
武漢工程大學(xué)化學(xué)與環(huán)境工程學(xué)院招收調(diào)劑生
+1/7
-
齊齊哈爾大學(xué)化學(xué)與化學(xué)工程學(xué)院招收專碩調(diào)劑
+1/7
-
南京林業(yè)大學(xué)化學(xué)工程學(xué)院柏惺峰課題組招收碩士研究生1名
+1/6
-
上海工程技術(shù)大學(xué)紡織服裝學(xué)院辛斌杰院長、鄭元生教授團(tuán)隊(duì)招收學(xué)碩/專碩研究生
+1/5
-
【光學(xué)工程研究生調(diào)劑】科研資源豐厚，歡迎優(yōu)秀學(xué)子加入
+1/4
-
長江師范學(xué)院化學(xué)化工學(xué)院2026年碩士研究生接收調(diào)劑的通知
+1/3
-
26年招收07 08學(xué)術(shù)型/專業(yè)型碩士研究生
+1/1
Mally89
鐵桿木蟲 (職業(yè)作家)
- 翻譯EPI: 79
- 應(yīng)助: 7 (幼兒園)
- 貴賓: 1.257
- 金幣: 5347.3
- 帖子: 3712
- 在線: 1225.5小時(shí)
- 蟲號(hào): 1150928
3樓2011-03-20 22:20:33
Mally89
鐵桿木蟲 (職業(yè)作家)
- 翻譯EPI: 79
- 應(yīng)助: 7 (幼兒園)
- 貴賓: 1.257
- 金幣: 5347.3
- 帖子: 3712
- 在線: 1225.5小時(shí)
- 蟲號(hào): 1150928
昔年殘夢(金幣+10, 翻譯EPI+1): 2011-03-20 22:44:44
|
Extracts for ACC synthase (ACS) activity assays were prepared according to the method of Boller et al. (1979), and determined by the method of Lizada and Yang (1979) with slight modifications. One g tomato pericarp tissue was homogenized using a cold pestle and mortar in 5mL of extraction buffer which consisting of 4 mM dithiothreitol, 10mM pyridoxal phosphate and 1.0mM EDTA in 100mM pH 8.0 Hepes buffer 根據(jù)1979年Boller 等進(jìn)行的ACC合成酶的提取物（ACS）的活性測定并經(jīng)Lizada和楊（1979）修改過后的準(zhǔn)備方法如下：將1g番茄果皮組織通過一個(gè)冷的杵和研缽進(jìn)行研磨，直到將其研磨成勻漿。在研磨過程中加入5毫升的提取緩沖液，其中包含有4毫摩二硫蘇糖醇，10毫摩磷酸吡哆醛，加有1毫摩EDTA的100毫摩、PH為8的HEPES緩沖液。 |
2樓2011-03-20 22:20:14
Mally89
鐵桿木蟲 (職業(yè)作家)
- 翻譯EPI: 79
- 應(yīng)助: 7 (幼兒園)
- 貴賓: 1.257
- 金幣: 5347.3
- 帖子: 3712
- 在線: 1225.5小時(shí)
- 蟲號(hào): 1150928
|
After centrifugation of the homogenate at 10,000×g for 20min, the supernatant was used for enzyme assays. All steps in the enzyme extraction were performed at 4◦C. ACC synthase activity was assayed in a 0.8mL reaction mixture containing 0.25mM S-adenosylmethionine, 50mM Hepes buffer and 10mM pyridoxal phosphate with 0.2mL of enzyme extract in test tubes. 將上述勻漿在10000轉(zhuǎn)20分鐘離心后，取上清液用于酶檢測。整個(gè)酶提取過程都應(yīng)該在4度環(huán)境下進(jìn)行。 ACC合成酶的活性需要放入裝有0.8mL的反應(yīng)復(fù)合物的試管中進(jìn)行測定，此復(fù)合物含0.25毫摩S -腺苷甲硫氨酸，50毫摩HEPES緩沖液和10毫摩帶有0.2ml酶提取物的磷酸吡哆醛。 |
5樓2011-03-20 22:27:26
Mally89
鐵桿木蟲 (職業(yè)作家)
- 翻譯EPI: 79
- 應(yīng)助: 7 (幼兒園)
- 貴賓: 1.257
- 金幣: 5347.3
- 帖子: 3712
- 在線: 1225.5小時(shí)
- 蟲號(hào): 1150928
6樓2011-03-20 22:28:10
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 材料科學(xué)與工程求調(diào)劑 +5 | 深V宿舍吧 2026-03-29 | 5/250 |
|
|---|---|---|---|---|
|
|
[考研] 化學(xué)0703 調(diào)劑 306分 一志愿211 +6 | 26要上岸 2026-03-28 | 6/300 |
|
|
|
[考研] 277跪求調(diào)劑 +6 | 1915668 2026-03-27 | 10/500 |
|
|
|
[考研] 各位老師好，我的一志愿為北京科技大學(xué)085601材料專碩 +8 | Koxui 2026-03-28 | 8/400 |
|
|
|
[考研] 085701求調(diào)劑初試286分 +4 | secret0328 2026-03-28 | 4/200 |
|
|
|
[考研] 312，生物學(xué)求調(diào)劑 +3 | 小譯同學(xué)abc 2026-03-28 | 3/150 |
|
|
[考研] 317求調(diào)劑 +6 | 十閑wx 2026-03-24 | 6/300 |
|
|
|
[考研] 0703本科鄭州大學(xué)求調(diào)劑 +3 | nhj_ 2026-03-25 | 3/150 |
|
|
|
[考研] 085701環(huán)境工程，267求調(diào)劑 +16 | minht 2026-03-26 | 16/800 |
|
|
|
[考研] 調(diào)劑 +3 | 好好讀書。 2026-03-28 | 3/150 |
|
|
|
[考研] 一志愿南京航空航天大學(xué)材料學(xué)碩求調(diào)劑 +3 | @taotao 2026-03-28 | 3/150 |
|
|
|
[考研] 340求調(diào)劑 +5 | jhx777 2026-03-27 | 5/250 |
|
|
|
[考研] 0703化學(xué)求調(diào)劑，各位老師看看我�。�！ +5 | 祁祺祺 2026-03-25 | 5/250 |
|
|
|
[考研] 085600，材料與化工321分，求調(diào)劑 +9 | 大饞小子 2026-03-27 | 9/450 |
|
|
|
[考研] 348求調(diào)劑 +4 | 小懶蟲不懶了 2026-03-27 | 5/250 |
|
|
|
[考研] 281求調(diào)劑 +3 | 亞克西good 2026-03-26 | 5/250 |
|
|
|
[考研] 總分322求生物學(xué)/生化與分子/生物信息學(xué)相關(guān)調(diào)劑 +5 | 星沉uu 2026-03-26 | 6/300 |
|
|
|
[考研] 289求調(diào)劑 +17 | 碩星赴 2026-03-23 | 17/850 |
|
|
|
[考研] 一志愿國科過程所081700，274求調(diào)劑 +3 | 三水研0水立方 2026-03-23 | 3/150 |
|
|
|
[考研] 284求調(diào)劑 +3 | yanzhixue111 2026-03-23 | 6/300 |
|