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[交流]
【求助/交流】求問從西格瑪買的連霉親和素修飾的金納米粒子怎么用
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我是學(xué)化學(xué)的,剛從sigma買了鏈霉親和素capped金納米粒子,,說明書上說稀釋了才能用,但是稀釋的具體操作我看不懂,外行真可悲。。。 哪位蟲友知道請指點(diǎn)一下 This product should be diluted for most applications. It is recommended that the diluent buffer contain 0.15 M saline buffered at pH 6 to 8, with 0.5% albumin (Product No. A 7638) and 0.05% TWEEN 20 to minimize background (additional buffer supplement may be required for certain applications e.g., see "dot blot" diluent). It is also recommended that prior to application, the diluted conjugate be allowed to equilibrate at least 20 minutes in lower glycerol content. Optimum concentration of the conjugate must be determined empirically dependent on specific usage and generally may range from final A 520 = 1.0 to 0.05 (1:2.5-1:50 dilution) with incubation times ranging from 30 minutes to 12 hours. |
» 搶金幣啦!回帖就可以得到:
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