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【分享】RNA介導(dǎo)的核體成核作用
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Nucleation of nuclear bodies by RNA RNA介導(dǎo)的核體成核作用 Sergey P. Shevtsov1 & Miroslav Dundr Nature Cell Biology Volume: 13, Pages: 167–173 Year published: (2011) DOI: doi:10.1038/ncb2157 Received 05 May 2010 Accepted 06 December 2010 Published online 16 January 2011 摘要:The biogenesis of the many functional compartments contained in the mammalian cell nucleus is poorly understood. More specifically, little is known regarding the initial nucleation step required for nuclear body formation. Here we show that RNA can function as a structural element and a nucleatorof nuclear bodies. We find that several types of coding and noncodingRNAs are sufficient to de novoassemble, and are physiologically enriched in, histonelocus bodies (with associated Cajalbodies), nuclear speckles, paraspecklesand nuclear stress bodies. Formation of nuclear bodies occurs through recruitment and accumulation of proteins resident in the nuclear bodies by nucleating RNA. These results demonstrate that transcription is a driving force in nuclear body formation and RNA transcripts can function as a scaffold in the formation of major nuclear bodies. Together, these data suggest that RNA-primed biogenesis of nuclear bodies is a general principle of nuclear organization. 1.哺乳動物細(xì)胞核包含的功能組分的生物起源并不清楚。更具體的說,關(guān)于核體形成所需的起始成核步驟知之甚少。這里,我們展示了RNA可以行使結(jié)構(gòu)元件的功能并且作為核體的成核劑。 2.我們發(fā)現(xiàn)幾類編碼和非編碼RNA就足以從頭組裝組蛋白體(與Cajal氏體相關(guān))、核斑、旁斑和壓力體(nSB),并且在生理狀況下它們中富集。核體的形成通過成核RNA募集并使蛋白在核體中積累。 3.這些結(jié)果闡釋轉(zhuǎn)錄是核體形成的驅(qū)動力且RNA轉(zhuǎn)錄物能行使主要核體形成支架的功能?偠灾@些數(shù)據(jù)暗示一個細(xì)胞核組織的一般原則:以RNA為本的核體的生物起源。 [ Last edited by silicare on 2011-4-1 at 18:04 ] |
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