我PCR后跑膠,目的產(chǎn)物的條帶都很正常,但是內(nèi)參照β-actin的條帶卻不正常。我用的β-actin分子量是330bp,但是跑出來(lái)的條帶卻還不到300bp,也就280-290左右。
請(qǐng)教大家這是為什么?
難道是我合成的引物有問(wèn)題?還是我跑膠有問(wèn)題,我跑了兩次了啊,結(jié)果都是這樣。
PS:這是我在NCBI上查到的我的β-actin信息,大俠們幫忙看一下對(duì)不對(duì)哈。
http://www.ncbi.nlm.nih.gov/genome/sts/sts.cgi?uid=271688
UniSTS:271688 Links
PMC18465P1
Bos taurus locus ACTB
Mus musculus chromosome 5, locus Actb
Rattus norvegicus locus Actb
Found by e-PCR in sequences from Bos taurus, Cricetinae gen. sp., Cryptosporidium muris, Mus musculus, Plasmodium yoelii, Rattus norvegicus and Zea mays.
Primer Information
Forward primer: CGTGGGCCGCCCTAGGCACCA
Reverse primer: TTGGCCTTAGGGTTCAGAGGGG
PCR product size: 330 (bp), Mus musculus
330 (bp), Rattus norvegicus
[ Last edited by waldenpond on 2011-4-6 at 17:11 ] |