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抗菌肽(antibacterial peptides)是一種由微生物、植物、無脊椎動物和各種動物的細胞和組織產(chǎn)生的一種抗菌活性多肽,也是生物體內(nèi)先天免疫系統(tǒng)中的重要組成部分。因具有獨特的抗菌機理有望成為替代抗生素成為新一代的抗微生物新藥。但來源問題始終是抗菌肽研究和利用的瓶頸,為了解決抗菌肽的來源問題,本文通過生物工程的技術和方法在大腸桿菌中成功表達出了有抗菌活性的抗菌肽LNK-16。 首先利用生物信息的方法,在NCBI上找到抗菌活性高、抗菌譜廣和分子量小的含有13個氨基酸殘基具有線性結(jié)構牛的抗菌肽Indolicidin。為了實現(xiàn)抗菌肽Indolicidin在大腸桿菌中表達,通過在N端加入激肽釋放酶的氨基酸識別位點(PFR),形成新的多肽命名為LNK-16。人工合成新的多肽LNK-16,抗菌活性檢測表明仍具有抗菌活性。 |
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Antibacterial peptides are a kind of polypeptide with antimicrobial activity produced by microorganisms, plants, invertebrates, and various animal cells and tissues, whic h are important component of innate immune system of biosystem in vivo. Due to their distinct antibacterial mechanism of antibiosis, antibacterial peptides are expected to become the next generation of anti-microbial drugs. But the source of antibacterial peptides has always been the bottleneck of their researches and utilizations. In order to solve the problem of their source, this paper successfully expressed antimicrobial peptides LNK-16 with antimicrobial activity with bio-engineering techniques and methods. At first, with the methods of bioinformation, we found the cattle antibacterial peptide Indolicidin with high antibacterial activity, broad antibacterial spectrum, low molecular weight and linear structure in the database of NCBI, which containing 13 amino acid peptides. In order to achieve the expression of Indolicidin in E.coli, by adding amino acid recognition sites of kallikrein (PFR) at the N terminal,we synthesized the new peptide named LNK-16. The detection of antibacterial activity showed that LNK-16 also had antibacterial activity. |
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