 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 4 | 1/1 | 返回列表 |
| 查看: 1033 | 回復(fù): 3 | |||
| 本帖產(chǎn)生 1 個 翻譯EPI ，點擊這里進行查看 | |||
[求助]
請幫忙翻譯成一段英文
|
|||
|
將酸性蛋白酶酶解產(chǎn)物經(jīng)過SP-Sephadex C-25強陽離子交換色譜、Sephadex G-50凝膠過濾色譜和反相高效液相色譜等步驟，從鯊魚皮明膠中分離到一種分子量為1356.3Da的抗氧化肽Ke-ⅱ，當其濃度為125μg/ml時，對DPPH自由基的清除率能夠達到62.20%。 為了進一步分離更多的膠原多肽，又采用超濾技術(shù)對酸性蛋白酶酶解產(chǎn)物中分子量小于3500Da的的組分進行初分離并濃縮，凍干后繼續(xù)采用和之前相同的色譜手段進行分離，最后得到三個抗氧化活性最強的膠原多肽B-Ⅲa 、B-Ⅲc和B-Ⅲd。經(jīng)C18反相高效液相色譜鑒定，這三個多肽組分都基本表現(xiàn)為單一峰。接著，再通過ESI Q-TOF MS對它們進行鑒定，結(jié)果表明B-Ⅲa 、B-Ⅲc、B-Ⅲd分別為分子量為944.5、784.5以及812.5的多肽。其中，B-Ⅲd經(jīng)氨基酸N端測序，得知其為含10個氨基酸，序列為Gly-Thr-X-Gly-Ala-Y-Gly-Pro-Z-Gly（未完全公開）的多肽。 |
回復(fù)此樓» 猜你喜歡
281求調(diào)劑（0805）
已經(jīng)有9人回復(fù)
-
293求調(diào)劑
已經(jīng)有14人回復(fù)
-
0703化學(xué)336分求調(diào)劑
已經(jīng)有6人回復(fù)
-
304求調(diào)劑
已經(jīng)有9人回復(fù)
-
環(huán)境領(lǐng)域全國重點實驗室招收博士1-2名
已經(jīng)有3人回復(fù)
-
材料專碩306英一數(shù)二
已經(jīng)有10人回復(fù)
-
301求調(diào)劑
已經(jīng)有6人回復(fù)
-
一志愿天津大學(xué)化學(xué)工藝專業(yè)（081702）315分求調(diào)劑
已經(jīng)有7人回復(fù)
-
302求調(diào)劑
已經(jīng)有6人回復(fù)
-
26博士申請
已經(jīng)有3人回復(fù)
ljason
新蟲 (文學(xué)泰斗)
外剛內(nèi)柔之逍遙達子
- 翻譯EPI: 30
- 應(yīng)助: 404 (碩士)
- 金幣: 381.5
- 散金: 24720
- 紅花: 229
- 沙發(fā): 595
- 帖子: 78709
- 在線: 2205小時
- 蟲號: 904684
- 注冊: 2009-11-16
- 專業(yè): 分離過程
- 管轄: 化學(xué)化工區(qū)
【答案】應(yīng)助回帖
xukun1176(金幣+100, 翻譯EPI+1): 2011-04-19 10:22:28
| 第一段：Antioxidant Peptide Ke-ⅱ with a molecular weight of 1356.3Da was isolated from shark skin gelatin after the characterization SP-Sephadex C-25 strong cation exchange chromatography, Sephadex G-50 gel filtration chromatography and RP-HPLC of acid protease enzyme product. The scavenging ratio could reach 62.20% when its concentration was 125μg/ml. |
2樓2011-04-13 14:43:36
ljason
新蟲 (文學(xué)泰斗)
外剛內(nèi)柔之逍遙達子
- 翻譯EPI: 30
- 應(yīng)助: 404 (碩士)
- 金幣: 381.5
- 散金: 24720
- 紅花: 229
- 沙發(fā): 595
- 帖子: 78709
- 在線: 2205小時
- 蟲號: 904684
- 注冊: 2009-11-16
- 專業(yè): 分離過程
- 管轄: 化學(xué)化工區(qū)
【答案】應(yīng)助回帖
| In order to further separation more collagen peptides, acid hydrolysis products of molecular weight less than 3500Da was separated and concentrated by ultrafiltration technology. the three strongest antioxidant activity of the collagen peptide B-Ⅲ a, B-Ⅲ c and B-Ⅲ d was obtained via lyophilize and chromatographic separation. the three polypeptide components were performed a single peak when identification by RP-HPLC C18. the molecular weight of the peptide B-Ⅲ a, B-Ⅲ c and B-Ⅲ d were 944.5,784.5 and 812.5 obtained from ESI Q-TOF MS identification. The B-Ⅲ d was contained 10 amino acids detected by amino acid N terminal sequencing and the sequence was Gly-Thr-X-Gly-Ala-Y-Gly-Pro-Z-Gly (not fully open). |
3樓2011-04-13 15:03:42
qingkong0918
金蟲 (小有名氣)
晴空
- 應(yīng)助: 1 (幼兒園)
- 金幣: 1106.5
- 散金: 185
- 紅花: 6
- 帖子: 223
- 在線: 116.6小時
- 蟲號: 1123756
- 注冊: 2010-10-16
- 性別: MM
- 專業(yè): 神經(jīng)精神藥物藥理
【答案】應(yīng)助回帖
xukun1176(金幣+100): 2011-04-19 10:22:37
| The zymohydrolysised product of acid protease is processed by Strong cation exchange SP-Sephadex C-25 , gel filtration chromatography Sephadex G-50 and reversed-phase high-performance liquid chromatography，and anti-oxidant peptide is obtained，whose molecular weight is 1356.3Da。 Rate of clearing free radical DPPH can reach 62.20% when the concentration is 125μg/ml . In order to separate more collagenous polypeptide， those whose molecular weight are less than 3500Da are separated and condensed initially by hythseperfiltration, then are ayophilized，seperated by the same chromatographs as above, three collagenous polypeptides with the most strong anti-oxidant activity are obtained, B-Ⅲa 、B-Ⅲc和B-Ⅲd. Assessed by C18 reversed-phase high-performance liquid chromatography , the three polypeptides all present simple spike. The molecular weight of the three polypeptides are 944.5、784.5 and 812.5 respectively. Especially , B-Ⅲd is assessed to have 10 amino acids , N terminal amino acid sequence is Gly-Thr-X-Gly-Ala-Y-Gly-Pro-Z-Gly（not fully publicized）. |
4樓2011-04-13 15:37:38
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 xukun1176 的主題更新
| 4 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 085601材料工程專碩求調(diào)劑 +5 | 慕寒mio 2026-03-16 | 5/250 |
|
|---|---|---|---|---|
|
|
[考研] 能源材料化學(xué)課題組招收碩士研究生8-10名 +3 | 脫穎而出 2026-03-16 | 6/300 |
|
|
[考研] 299求調(diào)劑 +4 | △小透明* 2026-03-17 | 4/200 |
|
|
|
[考研] 085601求調(diào)劑 +4 | Du.11 2026-03-16 | 4/200 |
|
|
|
[考研] 梁成偉老師課題組歡迎你的加入 +8 | 一鴨鴨喲 2026-03-14 | 10/500 |
|
|
|
[考研] 工科材料085601 279求調(diào)劑 +3 | 困于星晨 2026-03-17 | 3/150 |
|
|
|
[考研] 275求調(diào)劑 +4 | 太陽花天天開心 2026-03-16 | 4/200 |
|
|
|
[考研] 283求調(diào)劑 +3 | 聽風就是雨； 2026-03-16 | 3/150 |
|
|
|
[考研] 286求調(diào)劑 +3 | lemonzzn 2026-03-16 | 5/250 |
|
|
|
[考研] 311求調(diào)劑 +5 | 26研0 2026-03-15 | 5/250 |
|
|
|
[考研] 327求調(diào)劑 +6 | 拾光任染 2026-03-15 | 11/550 |
|
|
|
[考研] 085601材料工程315分求調(diào)劑 +3 | yang_0104 2026-03-15 | 3/150 |
|
|
|
[考研] 中科大材料與化工319求調(diào)劑 +3 | 孟鑫材料 2026-03-14 | 3/150 |
|
|
|
[考研] 一志愿西南交大，材料專碩317求調(diào)劑 +5 | lx8568 2026-03-11 | 5/250 |
|
|
|
[考研] 材料與化工085600調(diào)劑求老師收留 +9 | jiaanl 2026-03-11 | 9/450 |
|
|
|
[碩博家園] 085600 260分求調(diào)劑 +3 | 天空還下雨么 2026-03-13 | 5/250 |
|
|
|
[考研] 一志愿211化學(xué)學(xué)碩310分求調(diào)劑 +8 | 努力奮斗112 2026-03-12 | 9/450 |
|
|
|
[考研] 土木第一志愿276求調(diào)劑，科研和技能十分豐富，求新興方向的導(dǎo)師收留 +3 | 土木小天才 2026-03-12 | 3/150 |
|
|
|
[考研] 化工學(xué)碩306求調(diào)劑 +9 | 42838695 2026-03-12 | 9/450 |
|
|
|
[考研] 283求調(diào)劑，材料、化工皆可 +8 | 蘇打水7777 2026-03-11 | 10/500 |
|