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葉下珠粗提物抑菌活性研究 葉下珠是一年生草本植物，具有平肝清熱、利尿解毒功效。常用于治療腸炎、痢疾、傳染性肝炎、腎炎水腫、尿路感染、小兒疳積、火眼目翳、口瘡頭瘡、無(wú)名腫毒，對(duì)乙肝病毒也有一定的抑制作用。本文用去離子水、甲醇、乙醇、丙酮、石油醚這5種提取劑對(duì)葉下珠干粉進(jìn)行提取得到葉下珠粗提物。把葉下珠粗提物配成藥液對(duì)大腸桿菌、傷寒沙門(mén)式菌、痢疾志賀氏菌、蠟狀芽孢桿菌、金黃色葡萄球菌進(jìn)行抑菌活性測(cè)定。結(jié)果表明，5種粗提物對(duì)5種細(xì)菌都有一定的抑制效果，水提取物對(duì)大腸桿菌的抑制效果較好，達(dá)到了54.20%；甲醇提取物對(duì)痢疾志賀氏菌的抑制率達(dá)到了87.85%；乙醇提取物對(duì)金黃色葡萄球菌的抑制率達(dá)到了85.00%；丙酮提取物對(duì)金黃色葡萄球菌的抑制率達(dá)到了72.56%；石油醚對(duì)蠟狀芽孢桿菌的抑制率達(dá)到了32.78%。甲醇提取物對(duì)這5種細(xì)菌的抑菌效果最好，都達(dá)到了50%以上，其中對(duì)痢疾志賀氏菌的抑制率高達(dá)87.85%。甲醇提取物對(duì)3種病原真菌的EC50分別為1.5779 mg/ml、1.0926 mg/ml、2.1155mg/ml。 |
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【答案】應(yīng)助回帖
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| Extracts of Phyllanthus Phyllanthus antimicrobial activity is an annual herb, with Pinggan heat, detoxification diuretic effect. Used in the treatment of enteritis, dysentery, infectious hepatitis, nephritis, edema, urinary tract infection, infantile malnutrition product, fire shade the eyes, mouth sores head sores, swelling of unknown drugs, and hepatitis B virus have a certain extent. This deionized water, methanol, ethanol, acetone, petroleum ether extraction of these 5 agents were on the dry powder of Phyllanthus Phyllanthus extracted crude extract. The crude extract of Phyllanthus paired liquid on Escherichia coli, Salmonella typhi-type bacteria, Shigella dysenteriae, Bacillus cereus, Staphylococcus aureus, antibacterial activity determination. The results showed that crude extracts of 5 of 5 has some inhibitory effect of bacteria, water extracts inhibited E. coli better, reaching 54.20 percent; methanol extracts of Shigella dysentery inhibition rate reached 87.85%; ethanol extract on the inhibition of Staphylococcus aureus rate reached 85.00%; acetone extract on the inhibition of Staphylococcus aureus rate reached 72.56%; petroleum ether inhibition of Bacillus cereus rate reached 32.78%. Methanol extracts of these bacteria on the antibacterial activity of 5 the best, have reached more than 50%, in which the Shigella dysentery inhibition rate of 87.85%. Methanol extracts of 3 pathogenic fungi and EC50 were 1.5779 mg / ml, 1.0926 mg / ml, 2.1155mg/ml. |
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