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[求助]
請(qǐng)大家?guī)兔Ψg一段關(guān)于DPP-IV活性測試的文字
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| 初篩時(shí)每個(gè)樣品單濃度設(shè)雙復(fù)孔，抑制率大于50%的樣品進(jìn)行假陽性排除實(shí)驗(yàn),確證為陽性的測定IC50值，測定時(shí)每個(gè)樣品梯度稀釋六個(gè)濃度，每個(gè)濃度設(shè)雙復(fù)孔。根據(jù)抑制率，應(yīng)用Xlfit軟件中的4 Parameter Logistic Model計(jì)算IC50 |
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【答案】應(yīng)助回帖
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sltmac(金幣+2): 鼓勵(lì)交流 2011-04-28 10:48:55
sltmac(金幣+2): 鼓勵(lì)交流 2011-04-28 10:49:25
sltmac(金幣+8): 歡迎常來~~ 2011-04-28 11:46:04
sltmac(金幣+2): 鼓勵(lì)交流 2011-04-28 10:48:55
sltmac(金幣+2): 鼓勵(lì)交流 2011-04-28 10:49:25
sltmac(金幣+8): 歡迎常來~~ 2011-04-28 11:46:04
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Single concentration of each sample was set double hole when screening preliminarily. Samples, which its inhibition rate of more than 50%,were taken false positive exclusion test.Confirmed the determination of IC50 values for the positive test.Gradient of each sample was diluted six concentration when determining.Each concentration was set a double hole.According to the rate of inhibition,IC50 was calculated by 4 Parameter Logistic Mode of Xlfit. |
2樓2011-04-27 15:49:00
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