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dong5705銀蟲 (初入文壇)
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[求助]
高分求助 微生物類 細(xì)菌生物膜 碩士研究生論文摘要 漢譯英 急
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提 要 目的：進(jìn)一步完善大腸桿菌和金黃色葡萄球菌細(xì)菌生物膜模型，建立完善的細(xì)菌生物膜模型方法；考察具有抗菌作用的中藥單體及人參總皂苷對(duì)細(xì)菌生物膜的作用；篩選出對(duì)細(xì)菌生物膜具有較強(qiáng)抑制作用的人參皂苷單體；并考察其與中藥單體聯(lián)合應(yīng)用對(duì)細(xì)菌生物膜的影響。 方法：利用掃描電子顯微鏡及激光共聚焦顯微鏡觀察細(xì)菌生物膜的形成及形態(tài)；以大腸桿菌及金黃色葡萄球菌生物膜為篩選模型，將中藥單體物質(zhì)、人參總皂苷、各人參皂苷等物質(zhì)與細(xì)菌及細(xì)菌生物膜共同培養(yǎng)，通過酶標(biāo)儀讀取的OD值來測定細(xì)菌及生物膜的生長能力，通過計(jì)算抑菌率來觀察各樣品對(duì)細(xì)菌及生物膜的抑制作用的大��；采用微量肉湯稀釋法篩選與人參皂苷單體聯(lián)合用藥對(duì)細(xì)菌及生物膜產(chǎn)生影響的其他中藥單體。 結(jié)果：通過掃描電子顯微鏡（SEM）及激光共聚焦掃描電子顯微鏡（CLSM）鏡下觀察的結(jié)果，確定大腸桿菌和金黃色葡萄球菌在培養(yǎng)24h時(shí)，形成生物膜；人參總皂苷對(duì)大腸桿菌及生物膜具有很強(qiáng)的抑制作用，對(duì)金黃色葡萄球菌及BBF有一定的抑制作用，但抑制作用較弱；篩選人參單體皂苷，得到Rb1、Rb2、Rb3、Rc、Rd及對(duì)大腸桿菌及細(xì)菌生物膜有很強(qiáng)的抑制作用；聯(lián)合用藥結(jié)果顯示：Rb2與阿魏酸聯(lián)合應(yīng)用，對(duì)大腸桿菌BBF產(chǎn)生一定協(xié)同抑制作用。 結(jié)論：通過SEM和CLSM實(shí)驗(yàn)，進(jìn)一步完善了細(xì)菌生物膜模型；通過中藥單體對(duì)細(xì)菌及生物膜的作用，進(jìn)一步驗(yàn)證了細(xì)菌生物膜模型作為篩選慢性感染性藥物模型的可行性。篩選出對(duì)細(xì)菌生物膜具有較強(qiáng)抑制作用的人參皂苷單體；也篩選得到阿魏酸與人參皂苷單體聯(lián)合應(yīng)用對(duì)BBF產(chǎn)生協(xié)同抑制作用，這為下一步人參及人參與其它藥物聯(lián)合應(yīng)用，治療慢性感染性疾病的深入開發(fā)研究，打下良好的基礎(chǔ)，并提供科學(xué)的依據(jù)。 關(guān)鍵詞： 大腸桿菌 金黃色葡萄球菌 生物膜 人參皂苷 |
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zuodg
鐵桿木蟲 (正式寫手)
有為青年
- 翻譯EPI: 180
- 應(yīng)助: 5 (幼兒園)
- 貴賓: 0.015
- 金幣: 6765.2
- 散金: 48
- 紅花: 18
- 帖子: 500
- 在線: 285.5小時(shí)
- 蟲號(hào): 1055187
- 注冊(cè): 2010-07-10
- 性別: GG
- 專業(yè): 外國語言
2樓2011-04-27 20:11:12
zuodg
鐵桿木蟲 (正式寫手)
有為青年
- 翻譯EPI: 180
- 應(yīng)助: 5 (幼兒園)
- 貴賓: 0.015
- 金幣: 6765.2
- 散金: 48
- 紅花: 18
- 帖子: 500
- 在線: 285.5小時(shí)
- 蟲號(hào): 1055187
- 注冊(cè): 2010-07-10
- 性別: GG
- 專業(yè): 外國語言
【答案】應(yīng)助回帖
★
sltmac(金幣+1): 謝謝交流~~ 2011-04-28 10:43:01
dong5705(金幣+48, 翻譯EPI+1): 很不錯(cuò)了 比我寫的好多了 呵呵 非常感謝 2011-04-28 10:56:45
sltmac(金幣+1): 謝謝交流~~ 2011-04-28 10:43:01
dong5705(金幣+48, 翻譯EPI+1): 很不錯(cuò)了 比我寫的好多了 呵呵 非常感謝 2011-04-28 10:56:45
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Abstract Purpose: Improve bacterial biofilm model of E.coli and Staphylococcus aureus, and establish protocol for excellent bacterial biofilm model. Investigate effect of antibacterial Chinese medicine monomer and ginsenoside on bacterial biofilm. Screen ginsenoside monomer which inhibits bacterial biofilm. Observe combined affect of ginsenoside and Chinese medicine monomer on bacterial biofilm. Method: Observe formation and morphology of bacterial biofilm with SEM and LSCM. With E.coli and Staphylococcus aureus as screening model of bacterial biofilm, coculture of bacterial biofilm with Chinese medicine monomers, ginsenosides and single ginsenoside, and measure growth capacity of bacteria and biofilm based on OD values from microplate reader. Observe inhibitory capacity of different samples on bacteria and biofilms through inhibition rate. Screen other Chinese medicine monomers which have combined effect on bacterial biofilm with ginsenoside by trace broth dilution method. Result: With the application of SEM and LSCM, it is observed that biofilm of E.coli and Staphylococcus aureus begin to generate after 24 hrs’ culture. Ginsenosides have strong inhibitory effect on E.coliu and its biofilm, while they only inhibit Staphylococcus aureus and its biofilm to some extent. RB1、Rb2、Rb3、Rc、Rd, which inhibit E.coliu and its biofilm greatly, were obtained after screening of ginsenoside monomers Combined medication shows, combination of RB2 and Ferulic acid have collaborative inhibition on E.coliu and its biofilm Conclusion: With application of SEM and CLSM, bacterial biofilm model is further improved. Bacterial biofilm model as screening model of chronic infection drugs is furthrt confirmed through investigation of impact of Chinese medicine monomers on bacterial and BBF. Ginsensides which inhibit bacterial and BBF greatly were obtained. In addition, ginsenoside which have collaborative effect with Ferulic acid was also obtained, which lays good foundation for further combined medicate with ginseng and treatment of chronic infection disease. Key words: E.coli Staphylococcus aureus biofilm ginsenoside 今晚不在狀態(tài)，再加上對(duì)生物膜不太熟悉，所以翻譯的不好請(qǐng)見諒啊 |
3樓2011-04-27 21:41:00
zuodg
鐵桿木蟲 (正式寫手)
有為青年
- 翻譯EPI: 180
- 應(yīng)助: 5 (幼兒園)
- 貴賓: 0.015
- 金幣: 6765.2
- 散金: 48
- 紅花: 18
- 帖子: 500
- 在線: 285.5小時(shí)
- 蟲號(hào): 1055187
- 注冊(cè): 2010-07-10
- 性別: GG
- 專業(yè): 外國語言
4樓2011-04-27 21:50:29
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