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dong5705

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[求助] 急+急，求助多糖類摘要翻譯

中文摘要

目的：制備不同分子量片段的銀耳多糖，對其進行理化性質(zhì)、化學結構的研究，并篩選出對抗腫瘤藥物具有減毒、升高白細胞作用的最佳活性多糖。
方法：正交試驗法考察銀耳多糖的提取條件，確定提取工藝；酸水解方法制備不同分子量片段的銀耳多糖，通過苯酚-硫酸法、間羥基聯(lián)苯法、Lorry法及BCA法對其進行理化性質(zhì)的研究，HPLC法確定其糖組成，并采用甲基化、GC-MS研究其中性糖糖苷鍵連接方式。對正常小鼠及荷瘤鼠采用靜脈注射給藥，觀察不同分子量、不同側鏈樣品對抗腫瘤藥物的減毒作用。
結果：正交試驗法得到銀耳多糖的最佳提取工藝為50倍量的水進行煎煮，每次三小時共煎煮三次；通過酸水解法得到20萬、3萬和4000三個不同分子量片段的銀耳多糖；其理化性質(zhì)測定結果為：20萬分子量片段中性糖含量約為76.5%, 酸性糖含量約為19.8%，蛋白含量約為9%；3萬分子量片段中性糖含量約為78.2%，酸性糖含量約為22.1%，蛋白含量約為6.2%；4000分子量片段中性糖含量約為83.1%，酸性糖含量約為22.3%，蛋白含量約為8.2%。HPLC法分析結果表明，三個不同分子量片段的銀耳多糖均由Man、Glu、Xyl和GlcA組成。GC-MS結果可判定不同分子量片段的銀耳多糖均以1→3連接的Man為主鏈，并含有少量的1→4連接的Xyl；通過對不同分子量片段的銀耳多糖構效關系及其藥理作用的的研究可知分子量為3萬的銀耳多糖片段對抗腫瘤藥物致外周血白細胞減少具有較好的升高白細胞的作用，
結論：本實驗通過對銀耳多糖的提取、理化性質(zhì)、構效關系的研究并結合藥理實驗，篩選出具有對抗腫瘤藥物減毒升白作用的銀耳多糖片段，不僅對銀耳多糖構效關系的總結及作用機理有明顯的推動作用，也對藥物開發(fā)具有重要意義。

關鍵詞：銀耳多糖；理化性質(zhì)；PMP衍生化；HPLC；GC-MS

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好好學習，天天向上

1樓 2011-04-28 09:25:53

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zuodg

鐵桿木蟲 (正式寫手)

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【答案】應助回帖

dong5705(金幣+50, 翻譯EPI+1): 謝謝 2011-04-28 22:06:48

Abstract
Purpose: Prepare tremella polysaccharide of different molecule weight, investigate their physiochemical and structural properties and screen polysaccharide of optimum activity which decreases toxicity of antitumor drugs and promotes function of leukocytes.
Method: Extraction technology was obtained through orthogonal experiment of tremella polysaccharide. Tremella polysaccharides of different molecular weight were prepared by acid hydrolysis, physiochemical properties of which were investigated through phenol sulfuric acid method, m-hydroxydiphenyl method, Lorry method and BCA method. Saccharide component was determined through HPLC, and methylation and GC-MS were used to confirm glycosidic bond pattern of neutral monosaccharides. Conduct intravenous injection of normal mice and tumor-bearing mice, and observe toxicity reducing ability of tremella polysaccharide with different molecular weights and branch types on antitumor drugs.
Result: Optimum extraction technology drawn from orthogonal experiment is described below. Boil samples with 50 times of water for 3 times, and each time for 3 hrs. Tremella polysaccharides of 200,000 Dalton, 30,000 Dalton and 4,000 Dalton were prepared through acid hydrolysis. Physiochemical properties were determined. Molecule of 200,0000 Dalton contains 76.5% of neutral saccharides, 19.8% of acid saccharides and 9% of proteins. Molecule of 30,000 Dalton contains 78.2% of neutral saccharides 22.1% of acid saccharides and 6.2% of proteins. Molecule of 4,000 Dalton contains 83.1% of neutral saccharides 22.3% of acid saccharides and 8.2% of proteins. HPLC analysis shows, the above mentioned three molecules are composed of Man, Glu, Xyl, as well as small amount of 1→4 linked Xyl. It can be concluded from structure-activity analysis and pharmacological actions of tremella polysaccharide that molecule of 30,000 Dalton promotes leukocytes better when treating antitumor drugs caused decrease of peripheral blood leukocytes.
Conclusion: This experiment investigates isolation, physiochemical properties, structure-activity relationship, and pharmacology of tremella polysaccharide. Sugar fractions of tremella polysaccharide which decreases toxicity of antitumor drugs and promotes leukocyte, were screened. Structure-activity relationship and function mechanism of tremella polysaccharide were summarized, which is of importance to drug development.
Key words: tremella polysaccharide, physiochemical properties, PMP derivation, HPLC analysis, GC-MS

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要活的出彩

2樓2011-04-28 22:01:43

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