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an777

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摘要：【目的】利用實(shí)驗(yàn)室所分離出的小葉女貞枝葉部粗提產(chǎn)物進(jìn)行黃嘌呤氧化酶活性抑制作用實(shí)驗(yàn)，提取分離其中的黃酮類化合物作初步的定性測(cè)定。為進(jìn)一步探究小葉女貞在抗衰老等作用方面奠定基礎(chǔ)【方法】通過煎煮法和柱層析的方法提取分離出小葉女貞中的粗提產(chǎn)物，對(duì)其進(jìn)行黃嘌呤氧化酶活性抑制實(shí)驗(yàn)，之后通過干柱層析法分離粗提產(chǎn)物中的各個(gè)組分，最后利用黃酮類化合物的顯色反應(yīng)對(duì)產(chǎn)物中的黃酮類化合物進(jìn)行定性分析�！窘Y(jié)果】小葉女貞枝葉部初提產(chǎn)物對(duì)黃嘌呤氧化酶活性的抑制效果不夠明顯，對(duì)其進(jìn)一步提取分離得到六種產(chǎn)物，通過顯色反映鑒定出其中兩種產(chǎn)物屬于黃酮類化合物�！窘Y(jié)論】小葉女貞枝葉部粗提產(chǎn)物作為黃嘌呤氧化酶活性抑制劑的開發(fā)前景不大，但可在DPPH自由基清除率、羥基自由基清除率以及超氧陰離子清除率進(jìn)行進(jìn)一步的研究,以期在小葉女貞枝抗氧化作用方面取得新的收獲。

[ Last edited by an777 on 2011-4-28 at 13:44 ]

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1樓 2011-04-28 13:14:30

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zuodg

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【答案】應(yīng)助回帖

an777(金幣+200, 翻譯EPI+1): 萬分感謝 2011-04-28 14:15:11

引用回帖:

Originally posted by an777 at 2011-04-28 13:14:30:
摘要：【目的】利用實(shí)驗(yàn)室所分離出的小葉女貞枝葉部粗提產(chǎn)物進(jìn)行黃嘌呤氧化酶活性抑制作用實(shí)驗(yàn)，提取分離其中的黃酮類化合物作初步的定性測(cè)定。為進(jìn)一步探究小葉女貞在抗衰老等作用方面奠定基礎(chǔ)【方法】通過煎煮法 ...

Abstract
Purpose: Crude extracts from leaves and stems of Ligustrum quihoui Carr. were subjected to analyze their inhibition effect on xanthine oxidase, and flavones were used to conduct primary qualitative research. Our work lays foundation for anti-aging research of Ligustrum quihoui Carr.
Method: Crude extracts of Ligustrum quihoui Carr. were obtained by boil and chromatography. Flavones components from crude extracts were tested for their inhibitory effect on xanthine oxidase. Dry-column chromatography was performed to separate each fraction of crude extracts. In the end flavones chromogenic reaction was carried out to characterize flavones qualitatively.
Result: Inhibitory effect of crude extracts from leaves and stems of Ligustrum quihoui Carr. on Xanthine oxidase was not obvious enough. Total six compounds were obtained from further isolation, of which two are characterized to be flavone through chromogenic reaction.
Conclusion: It doesn’t seem optimistic to develop crude extracts from leaves and stems of Ligustrum quihoui Carr. into inhibition ingredient on xanthine oxidase. Further research work might be elimination of free radicals DPPH, hydroxyl radicals and superoxide anion with our progress. It is expected crude extracts from leaves and stems of Ligustrum quihoui Carr. might play a rule in anti-oxidation.

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2樓2011-04-28 14:08:26

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