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yangsc.06b銅蟲 (小有名氣)
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[求助]
請幫忙翻譯下這段話
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| 土壤中微生物和多環(huán)芳烴（PAHs）降解功能基因群落多樣性的研究對PAHs污染土壤的微生物修復(fù)具有重要指導(dǎo)意義。土壤中PAHs降解功能基因和PAHs降解的標(biāo)志微生物含量高低和多樣性極大地影響了能否在土壤中篩選到PAHs高效降解菌株和能否利用生物刺激降解土壤中的PAHs。近年來，分子生物學(xué)的發(fā)展極大地?cái)U(kuò)展了我們對于微生物多樣性的認(rèn)識。分子生物學(xué)方法可獲得關(guān)于土壤微生物群落的更精細(xì)結(jié)構(gòu)信息，可更為全面的反映微生物區(qū)系組成狀況。本研究以某焦化廠場地土壤為研究對象，通過分析土壤PAHs含量和土壤理化性質(zhì)，并利用土壤DNA提取、普通PCR、實(shí)時(shí)定量PCR和T-RFLP等現(xiàn)代分子生物學(xué)手段研究土壤細(xì)菌的豐度和群落多樣性，探究土壤微生物豐度和群落多樣性變化的影響因素。在此基礎(chǔ)上，以深層PAHs污染土壤為對象，通過振蕩措施改善土壤供氧速率，考察供氧能否促進(jìn)深層土壤PAHs降解以及降解過程中土壤微生物豐度和細(xì)菌群落多樣性動態(tài)演變規(guī)律。 |
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zuodg
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2樓2011-05-27 11:04:37
zuodg
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3樓2011-05-27 11:05:47
zuodg
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【答案】應(yīng)助回帖
yangsc.06b(金幣+45, 翻譯EPI+1): 2011-05-27 11:55:53
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Study of soil microorganism and diversification of PAHs degradative functional gene community is of great instructive value to microbiological bioremediation of PAHs polluted soil. Contents and diverification of soil PAHs degradative functional genes and PAHs degradation featured microorganisms greatly determine isolation of PAHs degradative strains with high efficiencies, and bioremediation of PAHs polluted soils. Recently, molecular biology greatly broadens our knowlege on diversification of soil microorganisms. With molecular biology, we can get detailed information on community structures of soil microorganisms, as well as floristic compositions of microorganisms. Starting from polluted soils, sampled from a coking plant, aboundance and diversification of soil microorganisms were investigated through analysis of physiochemical properties and PAHs contents of polluted soil. Molecular techniques, such as soil DNA isolation, PCR, realtime PCR and T-RFLP were also applicated. Influential factors of aboundance of soil microorganisms and community diversification were discussed. With PAHs polluted deep soil,soil oxygen exchanging rate was improved by shake to investigate whether it can help degradate PAHs from deep soil. Meanwhile, evolution features of aboundance of soil microorganisms as well as dynamic community diverisification structures were investigated. |
4樓2011-05-27 11:40:36
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