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lgq101木蟲 (小有名氣)
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[求助]
求翻譯實(shí)驗(yàn)過程~~~謝謝
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| General Procedure for Monitoring Solid-Phase Reactions. An aliquot of vacuum-dried resin (4 mg) was swollen with THF (5 mL) for 10 min. A solution of 4-methoxybenzylalcohol (internal standard) (25 mM in THF, 1 eq) was added, followed by tetra-n-butylammonium fluoride (0.1 M in THF, 3 eq). After stirring for 2 h, the THF solution was recovered from the resin, and the solution concentrated by rotary evaporation. The residue was taken up in CH3CN (300 μL) and filtered through a Pasteur pipette with a 0.5-inch plug of normal phase silica gel over a 0.5-inch plug of reverse-phase silica gel. The plug was rinsed carefully with 3 mL CH3CN, and the crude cleavage products were recovered by rotary evaporation. HPLC analysis (ELSD) was used to determine the ratio of cleaved scaffold to internal standard, and the yield was calculated based on a calibration curve. Analysis by 1H-NMR was used to determine product ratios where appropriate. |
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zuodg
鐵桿木蟲 (正式寫手)
有為青年
- 翻譯EPI: 180
- 應(yīng)助: 5 (幼兒園)
- 貴賓: 0.015
- 金幣: 6765.2
- 散金: 48
- 紅花: 18
- 帖子: 500
- 在線: 285.5小時(shí)
- 蟲號(hào): 1055187
- 注冊: 2010-07-10
- 性別: GG
- 專業(yè): 外國語言
【答案】應(yīng)助回帖
★
ringzhu(金幣+1): O(∩_∩)O~ 2011-06-04 13:12:28
lgq101(金幣+10, 翻譯EPI+1): 謝謝~~~~ 2011-06-04 15:43:01
ringzhu(金幣+1): O(∩_∩)O~ 2011-06-04 13:12:28
lgq101(金幣+10, 翻譯EPI+1): 謝謝~~~~ 2011-06-04 15:43:01
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固相合成一般流程 大孔樹脂4mg真空干燥，5ml THF消化10min。4-甲氧基苯甲醇溶液用作內(nèi)參（25mM, THF溶解）,隨后加入4-正丁基氟化銨（0.1M,THF溶解）。攪拌2h，除去大孔樹脂中的THF溶液，懸蒸法濃縮THF溶液。300 ul乙腈溶解殘留物，取0.5 英寸正相硅膠插頭置于0.5英寸反向硅膠插頭之上，巴氏吸管過濾。插頭用3ml乙腈仔細(xì)清洗，初級(jí)裂解產(chǎn)物使用懸蒸回收。HPLC法測定裂解比例，根據(jù)平衡曲線計(jì)算得率。分析1H譜數(shù)據(jù)，評(píng)估適當(dāng)條件下的產(chǎn)物得率。 很多專業(yè)東西不懂，樓主多指教 |
2樓2011-06-04 13:06:03
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