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gary286木蟲 (正式寫手)
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[求助]
審稿人問細(xì)胞毒性。why the sample has less viability at 4h than 8h,24h?
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審稿人問細(xì)胞毒性。why the sample has less viability at 4h than 8h,24h? 無機(jī)材料用MTT法做細(xì)胞毒性,4小時(shí)的cell viability 明顯小于8小時(shí)和24小時(shí)的。審稿人問原因,審稿人很可能也不是搞生物的。我能簡(jiǎn)單回答是誤差嗎(experiment error)?本人對(duì)細(xì)胞毒性檢測(cè)不太了解,見笑了,希望大家?guī)兔聪,給點(diǎn)解釋。。 PS, 無機(jī)材料為生物相容性材料修飾的Fe3O4。 |
細(xì)胞實(shí)驗(yàn) |
木蟲 (正式寫手)
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多謝,敘述如下: The cytotoxicity study was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in MCF-7 cells. MCF-7 cells were maintained in DMEM media supplemented with 10% fetal bovine serum (FBS) (Gibco, Grand Island, NY., USA), 2 mM L-glutamine, 100 mg/ml streptomycin, and 100 U/ml penicillin. The cell lines were cultured in 35 mm cover glass-bottom dish (SPL Life Sciences, Seoul, South Korea) at 37 oC in a humidified atmosphere containing 5% CO2. The magnetite particles at concentrations of 0, 0.03, 0.3 mg/mL in DMEM medium were mixed with the cells, and the cells were incubated for 4 h, 8 h, 24 h, 48 h, and 96 h. Optical density of each sample was measured in a Dynatech MR7000 ELISA reader at 570 nm. The optical density of the media was proportional to the number of viable cells. Inhibition of proliferation was evaluated as a percentage of control growth. The results were expressed as an average over three nominally identical measurements. 0.03 4~96h 97.33263; 96.01693;96.71721;97.61354;95.56296 0.3 4~96h 89.94877; 93.27308;94.00933;94.81344;91.00256 |
至尊木蟲 (文壇精英)
木蟲 (正式寫手)
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