 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 5 | 1/1 | 返回列表 |
| 查看: 660 | 回復(fù): 2 | |||
| 本帖產(chǎn)生 1 個(gè) 翻譯EPI ，點(diǎn)擊這里進(jìn)行查看 | |||
| 當(dāng)前只顯示滿足指定條件的回帖，點(diǎn)擊這里查看本話題的所有回帖 | |||
[求助]
幾句話，先謝謝了
|
|||
|
1.通過(guò)用透明膠帶將20個(gè)梳齒封住從而變成一個(gè)大的梳齒. 2.從中切取2個(gè)小膠條（同時(shí)在原膠條相應(yīng)部位做好標(biāo)記），并將其中一膠條斜切成3段貼放在含有5 mM PNPG底物的0.8% 瓊脂糖凝膠中于55 °C 溫育20min后，滴加數(shù)滴0.5 M Na2CO3，而另一小膠條則放置于考馬斯亮藍(lán)溶液中染色 . 3.與 Lee等人的報(bào)道相似 糖酶是一類誘導(dǎo)酶，在菌株發(fā)酵培養(yǎng)過(guò)程中，添加淀粉類物質(zhì)、如麥芽糖等不僅有利于微生物的生長(zhǎng)，而且可提高酶分泌的能力，其中木薯淀粉糖化液表現(xiàn)最為明顯，最高酶轉(zhuǎn)苷活力和水解活力分別達(dá)到111和12U/ml，菌體干重為 2g/l。乳糖有利于菌體長(zhǎng)，但卻阻礙了酶的分泌；添加木糖時(shí)，微生物的生長(zhǎng)受抑制，酶的合成量極少，幾乎沒(méi)有或者阻遏酶的合成，其主要原因是該物質(zhì)不能誘導(dǎo)基因的表達(dá)，此外，該條件下限制了菌體生長(zhǎng)，從而導(dǎo)致酶產(chǎn)量的降低。 |
回復(fù)此樓» 猜你喜歡
一志愿大連理工大學(xué)材料求調(diào)劑
已經(jīng)有5人回復(fù)
-
材料與化工調(diào)劑一志愿大連海事085600，349
已經(jīng)有6人回復(fù)
-
一志愿中海洋320化學(xué)工程與技術(shù)學(xué)碩求調(diào)劑
已經(jīng)有4人回復(fù)
-
材料科學(xué)與工程調(diào)劑
已經(jīng)有11人回復(fù)
-
311求調(diào)劑一志愿合肥工業(yè)大學(xué)
已經(jīng)有4人回復(fù)
-
一志愿食品科學(xué)與工程083200求調(diào)劑
已經(jīng)有4人回復(fù)
-
材料科學(xué)與工程求調(diào)劑
已經(jīng)有10人回復(fù)
-
一志愿北化085600材料專碩275|有文章專利｜求調(diào)劑
已經(jīng)有11人回復(fù)
-
一志愿 南京航空航天大學(xué) ，080500材料科學(xué)與工程學(xué)碩
已經(jīng)有4人回復(fù)
-
085600材料與化工調(diào)劑
已經(jīng)有15人回復(fù)
Carena
鐵桿木蟲 (知名作家)
- 翻譯EPI: 94
- 應(yīng)助: 5 (幼兒園)
- 貴賓: 0.542
- 金幣: 6371.2
- 散金: 8657
- 紅花: 55
- 沙發(fā): 23
- 帖子: 5968
- 在線: 357.5小時(shí)
- 蟲號(hào): 1049282
- 注冊(cè): 2010-06-29
- 性別: MM
- 專業(yè): 外國(guó)語(yǔ)言
【答案】應(yīng)助回帖
|
3.與 Lee等人的報(bào)道相似 糖酶是一類誘導(dǎo)酶，在菌株發(fā)酵培養(yǎng)過(guò)程中，添加淀粉類物質(zhì)、如麥芽糖等不僅有利于微生物的生長(zhǎng)，而且可提高酶分泌的能力，其中木薯淀粉糖化液表現(xiàn)最為明顯，最高酶轉(zhuǎn)苷活力和水解活力分別達(dá)到111和12U/ml，菌體干重為 2g/l。乳糖有利于菌體長(zhǎng)，但卻阻礙了酶的分泌；添加木糖時(shí)，微生物的生長(zhǎng)受抑制，酶的合成量極少，幾乎沒(méi)有或者阻遏酶的合成，其主要原因是該物質(zhì)不能誘導(dǎo)基因的表達(dá)，此外，該條件下限制了菌體生長(zhǎng)，從而導(dǎo)致酶產(chǎn)量的降低。 3. Similar to the reports by Lee et al which claimed that enzyme was a kind of sugar-induced enzyme. During the process of the fermental cultivation of strains, if starch substances, such as maltose, were added, the ability of enzyme secretion would be improved, among which cassava starch saccharification liquid was the most striking one, with its enzyme-turn-glycosides activity and hydrolysis activity reaching 111 and 12U/ml respectively and its dried cell of 2g/l. Lactose was conducive to cell length, but it hindered the enzyme secretion. When xylose was added, the growth of microorganisms was inhibited and there was hardly any synthesis of enzyme which might due to the fact that this substance could not induce gene expression. In addition, the cell growth was limited under the conditions which resulted in the low production of enzyme |
3樓2011-07-31 21:00:36
Carena
鐵桿木蟲 (知名作家)
- 翻譯EPI: 94
- 應(yīng)助: 5 (幼兒園)
- 貴賓: 0.542
- 金幣: 6371.2
- 散金: 8657
- 紅花: 55
- 沙發(fā): 23
- 帖子: 5968
- 在線: 357.5小時(shí)
- 蟲號(hào): 1049282
- 注冊(cè): 2010-06-29
- 性別: MM
- 專業(yè): 外國(guó)語(yǔ)言
【答案】應(yīng)助回帖
★ ★ ★ ★ ★
sltmac(金幣+5): 辛苦~~ 2011-07-31 21:45:19
qwky2010(金幣+66, 翻譯EPI+1): 謝謝了 2011-08-01 11:17:05
sltmac(金幣+5): 辛苦~~ 2011-07-31 21:45:19
qwky2010(金幣+66, 翻譯EPI+1): 謝謝了 2011-08-01 11:17:05
|
1.通過(guò)用透明膠帶將20個(gè)梳齒封住從而變成一個(gè)大的梳齒. 20 combs were bounded by scotch tapes to form one big comb 2.從中切取2個(gè)小膠條（同時(shí)在原膠條相應(yīng)部位做好標(biāo)記），并將其中一膠條斜切成3段貼放在含有5 mM PNPG底物的0.8% 瓊脂糖凝膠中于55 °C 溫育20min后，滴加數(shù)滴0.5 M Na2CO3，而另一小膠條則放置于考馬斯亮藍(lán)溶液中染色 2. 2 small strips then were cut at random (and made corresponding marks in the original tapes), and cut one of the two into 3 smaller ones and had them affixed on the 0.8% agarose gel with 5 mM PNPG substrate and incubated at 55 C for 20 min before dropping a few drops of 0.5 M Na2CO3. We then placed the other strip in the solution of Coomassie brilliant blue staining. |
2樓2011-07-31 20:37:05
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 277跪求調(diào)劑 +8 | 1915668 2026-03-27 | 12/600 |
|
|---|---|---|---|---|
|
[考研] 281求調(diào)劑 +5 | 亞克西good 2026-03-26 | 7/350 |
|
|
|
[考研] 085600 295分求調(diào)劑 +7 | W55j 2026-03-30 | 9/450 |
|
|
|
[考研] 332求調(diào)劑 +14 | 032500 2026-03-25 | 14/700 |
|
|
|
[考研] 367求調(diào)劑 +5 | 芋泥啵�！� 2026-03-28 | 5/250 |
|
|
|
[考研] 085601材料工程找調(diào)劑 +17 | oatmealR 2026-03-29 | 18/900 |
|
|
|
[考研] 284求調(diào)劑 +14 | junqihahaha 2026-03-26 | 15/750 |
|
|
|
[考研] 求調(diào)劑 +7 | 青春裁為三截 2026-03-29 | 7/350 |
|
|
|
[考研] 南京大學(xué)化學(xué)調(diào)劑 +10 | 景隨風(fēng) 2026-03-29 | 15/750 |
|
|
|
[考研] 311求調(diào)劑 +10 | lin0039 2026-03-26 | 10/500 |
|
|
|
[考研] 337求調(diào)劑 +6 | 《樹(shù)》 2026-03-29 | 6/300 |
|
|
|
[考研] 085600，材料與化工321分求調(diào)劑 +10 | 大饞小子 2026-03-28 | 10/500 |
|
|
|
[考研] 070305高分子化學(xué)與物理 304分求調(diào)劑 +12 | c297914 2026-03-28 | 12/600 |
|
|
|
[考研] 330分求調(diào)劑 +5 | qzenlc 2026-03-29 | 5/250 |
|
|
|
[考研] 283求調(diào)劑 +3 | A child 2026-03-28 | 3/150 |
|
|
|
[考研] 調(diào)劑 +3 | 好好讀書。 2026-03-28 | 3/150 |
|
|
|
[考研] 348求調(diào)劑 +4 | 小懶蟲不懶了 2026-03-27 | 5/250 |
|
|
|
[考研] 材料求調(diào)劑 +5 | .m.. 2026-03-25 | 5/250 |
|
|
|
[考研] 一志愿 南京郵電大學(xué) 288分 材料考研 求調(diào)劑 +3 | jl0720 2026-03-26 | 3/150 |
|
|
|
[考研] 機(jī)械學(xué)碩總分317求調(diào)劑�。。�！ +4 | Acaciad 2026-03-25 | 4/200 |
|