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小木蟲論壇-學術科研互動平臺 » 生物醫(yī)藥區(qū) » 微生物 » 實驗/技術 » （圖）這是什么儀器？
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jayzhao520木蟲 (正式寫手)
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（圖）這是什么儀器？ 已有2人參與
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這是我跟文獻作者要的一個 Sterile stirred batch culture fermentation vessels的圖片，不知道到底是啥，推測是Electrolab這個公司的生物反應器 下面是文獻里涉及的一部分內(nèi)容： 2.3. Faecal batch culture fermentation The fermentation profile of the freeze dried digested different sized oat flakes, the prebiotic oligofructose (Raftilose P95 ORAFTI) and the lowly fermented control carbohydrate cellulose (OXOID) was determined using anaerobic, pH controlled faecal batch cultures. Sterile stirred batch culture fermentation vessels (200 mL working volume) were prepared and aseptically filled with 180 mL of sterile basal nutrientmedium. Basalmedium contained per litre: 2 g Peptone, 2 g Yeast extract, 0.1 g NaCl, 0.04 g K2HPO4, 0.04 g KH2PO4, 0.01 g MgSO47H20, 0.01 g CaCl26H2O, 2 g NaHCO3,2ml Tween 80, 0.05 g Hemin dissolved in 1 ml of 4 M NaOH 10 ml Vitamen K (SIGMA), 0.5 g L-Cysteine HCL, and 0.5 g Bile Salts (sodium glycocholate and sodium taurocholate). The medium was adjusted to pH 7.0 and 4 mL of 0.025% (w/v) resazurin solution added prior to autoclaving. Once in the fermentation vessels, the sterilemediumwas sparged with O2-free N2 (15mL/min) overnight to maintain anaerobic conditions. The following day test substrates were allowed to dissolve in the basal medium to give a final concentration of 1% (w/v) before inoculation of the vesselswith 10% (w/v) faecal slurry, which was prepared with pre-reduced sterile phosphate-buffered saline (PBS, pH 7.0). Faecal samples were collected fromthree healthy faecal donors. All donors were healthy males aged between 23 and 31, and had not received antibiotic treatment for at least 3 months prior to stool collection, had not consumed pre- or probiotic supplements immediately prior to experimentation, and had no history of bowel disorders. The temperature of the fermentation vessels was held at 37 C by use of a circulating water bath, pH values were held between 6.7 and 6.8 by the addition of 0.5 M NaOH or HCl to the vessels, pH was controlled via pH meter controllers (Electrolab260, UK) and anaerobic conditions weremaintained by sparging the vessels with O2-free N2 (15 mL/min). A 5 ml sample was taken from each vessel immediately for analysis similarly samples were taken at 5, 10, and 24 h for analysis of SCFA by gas chromatography (GC) and for analysis of bacterial populations by fluorescence in situ hybrid- isation (FISH). This experiment was performed in triplicate. 求大神解答 |
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