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西瓜榮譽版主 (知名作家)
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分子生物版【Journal Club】預(yù)告：★第1講★8月16日:磁性納米材料用于核酸的分離純化 已有25人參與
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第1講已經(jīng)正式開講，請移步：http://www.gaoyang168.com/bbs/viewthread.php?tid=3490537&fpage=1 有興趣的蟲子可到活動主貼跟帖報名：http://www.gaoyang168.com/bbs/viewthread.php?tid=3468054&fpage=0&view=&highlight=&page=1 Contributions of Phosphate to DNA Adsorption/Desorption Behaviors Tanaka T, Sakai R, Kobayashi R, Hatakeyama K, Matsunaga T 【開講時間】：2011年8月16日 19:00 【內(nèi)容簡介】：主要給大家介紹下帶有氨基基團的磁性納米材料用于核酸的分離純化。屆時主持人將在分子生物版開貼給大家講解，歡迎大家到時回帖交流，主持人一周之內(nèi)每天都會抽時間回帖。 Abstract： The adsorption and desorption behaviors of DNA on aminosilane-modified magnetic nanoparticles were investigated by altering both type of anions and solvation state to achieve eficient recovery of DNA useful for subsequent polymerase chain reaction (PCR) analysis. The effects of multiple anions in accordance with the Hofmeister ion series were determined to clarify the contribution of phosphate ions on the effective desorption of DNA from aminosilane surfaces. Efficient DNA desorption (85% recovery) occurred in the presence of 1 M phosphate buffer, however, little DNA desorption was observed using any other anions. This phenomenon indicates that desorption originated from the replacement of DNA by phosphate ions. Furthermore, the adsorption and desorption were significantly affected by the addition of both protic and aprotic solvents. Efficient recovery of adsorbed DNA was attained using deoxynucleotide triphosphates (dNTPs) in place of phosphate buffer and was suitable for subsequent PCR analyses. Therefore, the DNA adsorption/desorption process proposed in this study will be a promising, novel approach for DNA purification with a high recovery ratio that is suitable for subsequent enzymatic reactions, such as PCR or restriction enzyme digestion.[ Last edited by 西瓜 on 2011-8-16 at 18:35 ] |
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