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huojinlong8610金蟲 (小有名氣)
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The signal peptide prediction performed by SignalP 3.0 on the basis of a combination of several artificial neural networks and hidden Markov models revealed that BMI RBKS contained a potential signal peptide with 58.8% probability. However, we search signal peptide using human and cattle RBKS protein sequences by SignalP 3.0, there is not a singal peptide found, the probability is low 1%. The reason about difference and conflict of pig , human and cattle RBKS signal peptide need further study later. RBKS was discovered more than 50 years ago, and there has been much progress in understanding this important enzyme within the last decade. But the molecular mechanisms of which actors and how target it remain a central unresolved problem for biochemists and pharmacologists. For this reason, the homology modeling of the BMI RBKS protein was carried out using human ribokinase (2fv7 Chain: B) as the template. Our results indicate that the conserved domain (RBKS, 17-323AA) of pig RBKS protein mainly exists αβα sandwich domain and β-barrel. It suggests that these domains may play a pivotal role in RBKS activity. This first pig structural model of RBKS should prove useful reference in designing studies aimed at understanding how RBKS interacts with unknown protein partners for explaining its various functions. , |
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huojinlong8610
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4樓2011-09-01 10:25:57
hefang2005
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先知
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sltmac(金幣+2): 辛苦 2011-09-01 10:44:32
sltmac(金幣+15): 盡管沒按樓主要求，還是給點(diǎn)辛苦費(fèi)，歡迎常來~~ 2011-09-07 07:47:48
sltmac(金幣+2): 辛苦 2011-09-01 10:44:32
sltmac(金幣+15): 盡管沒按樓主要求，還是給點(diǎn)辛苦費(fèi)，歡迎常來~~ 2011-09-07 07:47:48
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SignalP 3.0 Server 是計(jì)算機(jī)輔助預(yù)測蛋白質(zhì)位置的軟件版本。 基于幾種人工神經(jīng)網(wǎng)絡(luò)與隱藏的馬克模型，利用SignalP 3.0 系統(tǒng)預(yù)測的信號肽顯示出 BMI RBKS蛋白包含一個(gè)潛在的具有58.8%的概率存在的信號肽。然而，我們使用SignalP3.0搜尋了人類與牛RBKS 蛋白序列，沒法找到任何信號肽，其概率低至1%。此不同區(qū)別的原因以及豬、人、牛的RBKS 信號肽需要在將來進(jìn)一步研究。 RBKS蛋白在50年前就被發(fā)現(xiàn)，在最近10年對理解這種重要的酶取得了巨大進(jìn)步。但是其分子機(jī)制以及如何相應(yīng)信號對生物化學(xué)家與藥理學(xué)家而言仍然是一個(gè)沒有解決的核心問題。為此，我們使用ribokinase (2fv7 Chain: B)人類來源的酶作為模板，以創(chuàng)建BMI RBKS 蛋白的同源模型。我們的結(jié)果表明：豬RBKS 蛋白中的(RBKS, 17-323AA)區(qū)域存在一種αβα構(gòu)成的三明治結(jié)構(gòu)以及β-barrel，表明了這些區(qū)域在表達(dá)RBKS蛋白活性起著重要作用。第一個(gè)豬的RBKS模型為在設(shè)計(jì)研究時(shí)理解RBKS 如何與位置蛋白配體之間作用，以了解其各類功能特性將有可能提供有用的參考。 |
2樓2011-09-01 10:14:59
phu_grassman
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愛與雨下(金幣+1): 感謝你的支持！ 2011-09-01 15:37:23
huojinlong8610(金幣+10, 翻譯EPI+1): 2011-09-01 16:17:31
sltmac(金幣+35): 歡迎常來~~ 2011-09-07 07:48:00
愛與雨下(金幣+1): 感謝你的支持！ 2011-09-01 15:37:23
huojinlong8610(金幣+10, 翻譯EPI+1): 2011-09-01 16:17:31
sltmac(金幣+35): 歡迎常來~~ 2011-09-07 07:48:00
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The signal peptide prediction performed by SignalP 3.0 on the basis of a combination of several artificial neural networks and hidden Markov models revealed that BMI RBKS contained a potential signal peptide with 58.8% probability. However, （加when）we search（時(shí)態(tài)是不是應(yīng)該一致） signal peptide using human and cattle RBKS protein sequences by SignalP 3.0, there is not a singal peptide found, the probability is low 1%（as low as 1%）. The reason about (The reason for the )difference and conflict of pig , human and cattle RBKS signal peptide need further study later(needs further study, later可刪去). RBKS was discovered more than 50 years ago, and there has been much progress in understanding this important enzyme within the last decade. But the molecular mechanisms of which actors and how target it （這一句可否提供中文翻譯）remain a central unresolved problem for biochemists and pharmacologists. For this reason, the homology modeling of the BMI RBKS protein was carried out using human ribokinase (2fv7 Chain: B) as the template. Our results indicate that the conserved domain (RBKS, 17-323AA) of pig RBKS protein mainly exists（exist 為不及物動(dòng)詞，是不是以這兩種形式存在，可以在前面加in the form of） αβα sandwich domain and β-barrel. It suggests that these domains may play a pivotal role in RBKS activity. This first pig structural model of RBKS should prove useful reference in designing studies aimed at understanding how RBKS interacts with unknown protein partners for explaining its various functions. , |
6樓2011-09-01 13:55:01
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