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bamage木蟲 (著名寫手)
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[求助]
求助一段生物學(xué)方面文獻(xiàn)的翻譯
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求助分子生物學(xué)方面的一段文獻(xiàn)翻譯，謝謝！ Determination of Distance Restraints from PREs PRE-derived distances were determined from methyl-TROSY spectra of SecA by measuring peak intensities before (paramagnetic) and after (diamagnetic) reduction of the nitroxide spin label with ascorbic acid. PRE values were then converted to distances by using a modified Solomon-Bloembergen equation for transverse relaxation, as described previously. Two sets of restraints were incorporated into subsequent structure calculations. Methyl groups strongly affected by the presence of the spin label in the peptide and whose resonances broaden beyond detection in the paramagnetic spectrum, were restrained with only an upper bound distance estimated from the noise of the spectrum plus 4 Å. Methyl groups whose resonances appear in the paramagnetic spectra were restrained as the calculated distance with ± 4 Å upper/lower bounds. [ Last edited by bamage on 2011-9-25 at 12:07 ] |
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fanliang1389
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有愛的二哥
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【答案】應(yīng)助回帖
★
愛與雨下(金幣+1): ！~ 2011-09-26 12:06:23
bamage(金幣+3, 翻譯EPI+1): 謝謝回帖！ 2011-09-27 18:00:33
bamage(金幣+5): 2011-09-27 20:34:23
bamage(金幣+12): 2011-09-29 17:04:39
愛與雨下(金幣+1): ！~ 2011-09-26 12:06:23
bamage(金幣+3, 翻譯EPI+1): 謝謝回帖！ 2011-09-27 18:00:33
bamage(金幣+5): 2011-09-27 20:34:23
bamage(金幣+12): 2011-09-29 17:04:39
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由PREs中檢測距離檢測限 由SecA中的甲-trosy光譜檢測PRE的導(dǎo)出距離，該檢測距離是通過檢測硝基氧自旋標(biāo)記物與抗壞血酸在減弱前（順磁性）和減弱后（逆磁性）來實(shí)現(xiàn)的。之后，采用更改過的Solomon-Bloembergen方程，將PRE值轉(zhuǎn)化為距離，采用該方程的目的在于橫向松弛，此方法之前已經(jīng)進(jìn)行了描述。兩組裝置結(jié)合為之后的結(jié)構(gòu)計(jì)算。受到肽中的自旋標(biāo)記物的強(qiáng)烈影響且其共振態(tài)加寬并超出順次共振譜的檢測范圍的甲基原子團(tuán)只受最大距離的限制，該距離由光譜的噪音+4A估算而出。在順磁光譜中可以看到甲基原子團(tuán)，其最大/最小檢測距離分別為計(jì)算值± 4 Å |
2樓2011-09-26 11:47:04
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