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wo357026239銅蟲 (正式寫手)
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[求助]
論文摘要翻譯(英譯中)
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Nanotechnology has been used to provide advanced biomedical research tools in diagnostic imaging and therapy, which requires targeting of nanoparticles (NPs) to individual cells and subcellular compartments. However, a complete understanding of the intracellular uptake, transport, and subcellular distribution of nanostructured materials remains limited. Hence, gold NPs were explored as a model system to study the intracellular behavior of NPs in real time. Our results show that the cellular uptake of gold NPs is dependent on their size and surface properties. The NPs were transported in vesicles of 300–500 nm diameter within the cytoplasm. The average velocity and diffusion coefficient of the vesicles containing NPs were 10.2 (±1.8) μm/hr and 3.33 (±0.52) μm2/hr, respectively. Analysis of the time-dependent intracellular spatial distribution of the NPs demonstrated that they reside in lysosomes (final degrading organelles) within 40 minutes of incubation. These findings can be used to tailor nanoscale devices for effective cell targeting and delivery. |
金蟲 (職業(yè)作家)
| 納米技術(shù)已經(jīng)被用作診斷影像和治療學(xué)中的高級(jí)生物醫(yī)學(xué)研究工具,這個(gè)工具能把納米粒子(Nps)靶向單獨(dú)的細(xì)胞或亞細(xì)胞器。然而對(duì)于納米材料如何在細(xì)胞內(nèi)的吸收、運(yùn)輸和亞細(xì)胞區(qū)的分配的知識(shí)非常有限。因此納米金顆粒作為模式體系去研究Nps的細(xì)胞內(nèi)行為的方法應(yīng)運(yùn)而生。我們的研究結(jié)果表明:細(xì)胞對(duì)于納米金顆粒的吸收依賴于顆粒的尺寸與表面特性。在胞質(zhì)中,Nps被包含在直徑300-500nm的囊泡中運(yùn)輸,其中囊泡的平均速率和擴(kuò)散系數(shù)分別為10.2 (±1.8) μm/hr 和 3.33(±0.52) μm2/hr。對(duì)于具有時(shí)間性的細(xì)胞內(nèi)Nps空間分布的分析表明,在孵化(incubation)40min之內(nèi),Nps存在于溶酶體(最終降解的細(xì)胞器)內(nèi)。這個(gè)發(fā)現(xiàn)能應(yīng)用于設(shè)置納米級(jí)的裝置,這樣就可以有效地進(jìn)行細(xì)胞靶向和轉(zhuǎn)運(yùn)。 |
榮譽(yù)版主 (文壇精英)
有愛的二哥
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納米技術(shù)已為診斷性影像學(xué)和療法提供了血多高級(jí)生物醫(yī)學(xué)研究工具,而診斷性療法需要納米粒直接命中人體細(xì)胞和亞細(xì)胞區(qū)室。然而,目前對(duì)細(xì)胞內(nèi)的攝入、轉(zhuǎn)移以及亞細(xì)胞間納米結(jié)構(gòu)材料的分布情況的了解仍然有限。因此,開發(fā)出金納米粒作為模型系統(tǒng)以研究其在細(xì)胞內(nèi)的表現(xiàn)刻不容緩。結(jié)果表明,細(xì)胞攝入金納米粒與其大小和表面屬性有關(guān)。納米粒在細(xì)胞質(zhì)內(nèi)的直徑在300-500nm之間的小囊泡間傳送。含有納米粒的小囊泡的平均速度為10.2 (±1.8) μm/hr,擴(kuò)散率為3.33 (±0.52) μm2/hr 對(duì)細(xì)胞內(nèi)空間分布的時(shí)間依賴性分析表明在40分鐘內(nèi)這些納米粒進(jìn)入了溶酶體內(nèi)(溶酶體為最終降解細(xì)胞器)。這些研究結(jié)果能夠?qū)⒓{米級(jí)設(shè)備用于高命中的細(xì)胞打靶和高效的細(xì)胞轉(zhuǎn)移中來。 |
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