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米西222新蟲(chóng) (初入文壇)
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[求助]
關(guān)于Dna transposon
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我是初學(xué)生物的新手 最近看英文有一段關(guān)于Dna transposon 的解釋看不懂 求高手解釋下：cut and paste 型的轉(zhuǎn)座怎么會(huì)有duplication呢？（最好簡(jiǎn)要說(shuō)下下面文字重點(diǎn)）不甚感激~~~~ Class II (DNA transposons): By contrast, the cut-and-paste transposition mechanisms of class II transposons do not involve an RNA intermediate. These transpositions are catalyzed by various types of transposase enzymes. Some transposases can bind non-specifically to any target site, while others bind to specific sequence targets. The transposase makes a staggered cut at the target site producing sticky ends, cuts out the transposon and ligates it into the target site. A DNA polymerase fills in the resulting gaps from the sticky ends and DNA ligase closes the sugar-phosphate backbone. This results in target site duplication and the insertion sites of DNA transposons may be identified by short direct repeats (a staggered cut in the target DNA filled by DNA polymerase) followed by inverted repeats (which are important for the transposon excision by transposase). The duplications at the target site can result in gene duplication, which plays an important role in evolution[4]:284. |
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