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binxman木蟲 (著名寫手)
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[求助]
80金幣求助英譯中，禁止機器翻譯，謝謝！
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A HEX-1 crystal lattice required for Woronin body function in Neurospora crassa. The Woronin body is a dense-core vesicle specific to filamentous ascomycetes (Euascomycetes), where it functions to seal the septal pore in response to cellular damage. The HEX-1 protein self-assembles to form this solid core of the vesicle. Here, we solve the crystal structure of HEX-1 at 1.8 A, which provides the structural basis of its self-assembly. The structure reveals the existence of three intermolecular interfaces that promote the formation of a three-dimensional protein lattice. Consistent with these data, self-assembly is disrupted by mutations in intermolecular contact residues and expression of an assembly-defective HEX-1 mutant results in the production of aberrantWoronin bodies, which possess a soluble noncrystalline core. This mutant also fails to complement a hex-1 deletion in Neurospora crassa, demonstrating that the HEX-1protein lattice is required for Woronin body function. Although both the sequence and the tertiary structure of HEX-1 are similar to those of eukaryotic initiation factor 5A (eIF-5A), the amino acids required for HEX-1 self-assembly and peroxisomal targeting are absent in eIF-5A. Thus, we propose that a new function has evolved following duplication of an ancestral eIF-5A gene and that this may define an important step in fungal evolution. |
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fuaixiang
金蟲 (小有名氣)
- 翻譯EPI: 23
- 應(yīng)助: 0 (幼兒園)
- 金幣: 1024.8
- 帖子: 56
- 在線: 27.6小時
- 蟲號: 1009478
- 注冊: 2010-05-01
- 專業(yè): 人體解剖與組織胚胎學
【答案】應(yīng)助回帖
binxman(金幣+80, 翻譯EPI+1): 謝謝！太感謝了！救命恩人啊 2011-10-21 23:09:45
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粗糙鏈孢菌中烏頭類生物堿功能所需的HEX-1晶格 烏頭類生物堿是一種有致密核的囊泡，對絲狀子囊菌（真子囊菌綱）有特效，其功能是封閉中隔孔以應(yīng)對細胞損傷。HEX-1蛋白自組裝而形成囊泡的實心核。在這里，我們在1.8 A處解開HEX-1的晶體結(jié)構(gòu)，1.8 A提供了其自組裝的結(jié)構(gòu)基礎(chǔ)。這種結(jié)構(gòu)顯示，有三個分子間接口促進三維蛋白晶格的形成。與這些數(shù)據(jù)一致的是，分子間接觸殘基的突變干擾了自組裝，自組裝缺陷HEX-1突變體的表達導致異常烏頭類生物堿（具有水溶性非晶核）的產(chǎn)生。這種突變體也不能補足粗糙鏈孢菌中的hex-1缺失，證明HEX-1蛋白晶格是烏頭類生物堿功能所必需的。盡管HEX-1的序列和三級結(jié)構(gòu)都與真核起始因子5A（eIF-5A）類似，但是eIF-5A中缺少HEX-1自組裝和過氧化物酶體定位所需的氨基酸。因此，我們認為，原始eIF-5A基因復制后形成了一種新功能，這可能決定了真菌進化中的一個重要步驟。 |
2樓2011-10-21 21:33:56
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