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wangjun007金蟲 (正式寫手)
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[求助]
介孔硅藥物釋放
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| 有誰做過介孔硅的藥物釋放,你們是怎么做的?我是用透析的方法,但發(fā)現(xiàn)有沉淀物在透析袋里,這樣藥物還能出來嗎? |
金蟲 (正式寫手)
金蟲 (正式寫手)
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For the pH dependent release studies, three different buffer solutions with the same NaCl concentration (0.15 M) were chosen: sodium acetate buffer for pH 5.0, phosphate buffer for pH 6.5 and 7.4. DOX@M-MSN–P(NIPAM-co-MAA) was dispersed in 2 mL of buffer, and the dispersion was transferred into a dialysis bag (molecular weight cut off ¼ 14 000). The dialysis bag was then kept in 200 mL of buffer and gently shaken at 37 C. At timed intervals, 1 mL of solution was withdrawn from the solution and analyzed by UV-vis. To keep a constant volume, 1 mL of fresh buffer was added after each sampling. All drug release results were averaged with three measurements,其實(shí)文獻(xiàn)挺多的,大致就是這么個(gè)方法,你要根據(jù)具體情況而定 |
金蟲 (正式寫手)
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