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gmy1990

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[求助] 急救��！生物傳感器--翻譯

single layer graphene ﬂakes were transferred to the surface of an oxidized Si substrate using mechanical exfoliation,source/drain contacts were then deﬁned by electron beam lithography (EBL) followed by Cr/Au/Cr metallization, and then the contacts were passivated using SiO2.SiNW-FETs were deﬁned close to Gra-FET devices in a second round of fabrication. Speciﬁcally, 30 nm diameter p-type SiNWs were deposited from ethanol solution onto the chip with Gra-FET devices, and then EBL and metallization steps were carried out to deﬁne source/drain electrodes.Last, the substrate chip was coated with a poly(methyl methacrylate) passiva-tion layer, and 50 µm × 50 µm windows were deﬁned to expose the Gra- and NW-FET devices. Gra- and SiNW-FET device chips were interfaced with embryonic chicken cardiomyocytes as shown schematically in Figure 1B. Nanodevice cardiomyocyte interfaces weremade in a ﬂexiblemanner using our recently reported scheme: First,embryonic chicken cardiomyocytes were cultured under optimized conditions on thin poly(dimethylsiloxane) (PDMS)
sheets;second, a PDMS/cardiomyocyte substrate was transferred into a well, which contains extracellular medium, over the Gra-FET and SiNW-FET chip; third, the PDMS/cardiomyocyte cell substrates were positioned using a x-y-z manipulator under an optical microscope to bring spontaneously beating cells into direct contact with devices (Figure 1B).
麻煩哪位大俠幫忙翻譯一下啊！不要機(jī)譯��！非常感謝

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1樓 2011-12-31 16:38:25

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xiaohanhan2007

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【答案】應(yīng)助回帖

gmy1990(金幣+80, 翻譯EPI+1): 2011-12-31 22:22:16

將單層石墨薄片通過機(jī)械剝離轉(zhuǎn)移到氧化硅襯底表面，并對(duì)源/漏接點(diǎn)進(jìn)行電子束光刻（EBL）修邊和Cr/Au/Cr金屬化，接著將這些接點(diǎn)使用SiO2進(jìn)行鈍化。之后對(duì)SiNW-FETs進(jìn)行的第二輪加工與GRA-FET加工方法相似。具體來說，將直徑30 nm的p-型SiNWs從乙醇溶液中沉積到連有通過Gra-FET器件的芯片上，接著對(duì)源/漏電極使用EBL和金屬化修邊。最后，將襯底芯片涂上聚（甲基丙烯酸甲酯）鈍化層，并切出50微米× 50 微米的窗口以便露出Gra和NW-FET器件。如圖1B所示，將Gra和NW-FET器件芯片連接胚胎雞心肌細(xì)胞。納米器件心肌細(xì)胞連接體可按照我們近期報(bào)道的方法靈活制得：首先，將胚胎雞心肌細(xì)胞在優(yōu)化條件下在聚二甲基硅氧烷（PDMS）薄片表面進(jìn)行培養(yǎng)；第二步，將一個(gè)PDMS/心肌細(xì)胞基底轉(zhuǎn)移到含有胞外液的槽中，并置于Gra-FET和SiNWet-FET芯片之上；第三步，將這一PDMS/心肌細(xì)胞基底通過x-y-x探針座置于光學(xué)顯微鏡下，并將自發(fā)性搏動(dòng)細(xì)胞與器件直接進(jìn)行接觸。

贊一下

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2樓2011-12-31 21:51:25

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