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phitong木蟲 (小有名氣)
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[求助]
土壤碳庫測定
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現(xiàn)在小蟲做實驗,欲采用Rovira and Vallejo(2002)的方法來分析土壤活性和惰性有機(jī)碳庫。 Rovira and Vallejo(2002)文章簡述如下:Briefly, 20 mL of 5NH2SO4 was added to 500 mg soil, and the sample was hydro- lyzed for 30 min at 105 C in sealed Pyrex tubes, after which the hydrolysate was recovered by centrifugation and decantation. The residue was washed with 20 mL of de-ionized water and the washing added to the hydrolysate. This hydrolysate was taken as labile pool I (LPI) and analyzed for labile pool I carbon (LPI-C) and nitrogen (LPI-N). The remaining residue was hydrolyzed with 2 mL of 26 N H2SO4 overnight at room temperature under continuous shaking. The concentration of the acid was then brought down to 2 N by dilution with de-ionized water and the sample was hydro- lyzed for 3 h at 105 Cwith occasional shaking. The hydrolysatewas recovered in the same manner as for the LPI. This second hydro- lysatewas taken as labile pool II (LPII) and analyzed for labile pool II carbon (LPII – C) and nitrogen (LPII – N). 問題如下:1.離心速度多大合適? 2.稱量土,加入2.5 M H2SO4溶液這一步可以直接放在離心管中高溫水解嗎?不可以的話,該用什么容器,之后如何無損失轉(zhuǎn)移? |
土壤固碳科學(xué) |
木蟲 (小有名氣)
木蟲 (小有名氣)
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