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[資源]
分享：核酸修飾石墨烯作生物感應(yīng)器
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 Abstract There is immense demand for complex nanoarchitectures based on graphene nanostructures in the fields of biosensing or nanoelectronics. DNA molecules represent the most versatile and programmable recognition element and can provide a unique massive parallel assembly strategy with graphene nanomaterials. Here we demonstrate a facile strategy for covalent linking of single stranded DNA (ssDNA) to graphene using carbodiimide chemistry and apply it to genosensing. Since graphenes can be prepared by different methods and can contain various oxygen containing groups, we thoroughly investigated the utility of four different chemically modified graphenes for functionalization by ssDNA. The materials were characterized in detail and the different DNA functionalized graphene platforms were then employed for the detection of DNA hybridization and DNA polymorphism by using impedimetric methods. We believe that our findings are very important for the development of novel devices that can be used as alternatives to classical techniques for sensitive and fast DNA analysis. In addition, covalent functionalization of graphene with ssDNA is expected to have broad implications, from biosensing to nanoelectronics and directed, DNA programmable, self-assembly. Graphene design: Four different chemically modified graphenes were produced, characterized and used for the covalent immobilization of DNA probes. The impedimetric detection of DNA hybridization on these platforms was compared (see illustration). The best sensitivity and reproducibility was obtained when using electrochemically reduced graphene oxide. This platform was employed for the detection of DNA single nucleotide polymorphism. |
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