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北京石油化工學院2026年研究生招生接收調劑公告

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[求助] 一篇生物文獻求翻譯呀

Isolation and Characterization of Four Forms of Kallikrein from Hog Pancreas Autolysate1
Four forms of kallikrein, designated as I-IV, were isolated from hog pancreas autolysate mainly by chromatographies on Lysine-aminohexyl-Sepharose and on DEAE-Sepharose CL-6B, with yields of 65, 56, 59, and 41 mg, respectively, from 10 kg of the tissue. They were homogeneous on polyacrylamide gel electrophoresis, indistinguishable from eath other immunologically and had the same amino acid composition. Kallikreins I, II, and III contained carbohydrate, but kallikrein IV was essentially carbohydrate free. On reduction with mercaptoethanol, each of them produced two polypeptide chains with different molecular weights. The H (heavy) chains from kallikreins I and II were identical, designated as Hj. It was a glycoprotein with apparent molecular weight of 21,000, whereas the H chain from III and IV, designated as H,, had a molecular weight of 17,000 and was regarded as an H! chain devoid of carbohydrate. Likewise, the L (light) chain from kallikreins I and III, designated as LL, was a glycoprotein with an apparent molecular weight of 12,000, whereas L, from II and IV corresponded to L^ devoid of carbohydrate and had a molecular weight of 8,500. Thus, kallikreins I-IV could be expressed as Hil^, HiL,, HJLJ, and H,Li, respectively. They had comparable specific activities and Km values towards synthetic substrates. The isoelectric points of kallikreins I-IV were nearly the same, with values of 4.0-4.1.

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1樓 2012-02-01 16:24:36

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【答案】應助回帖

qianlinguang(金幣+10, 翻譯EPI+1): ★★★很有幫助謝謝哦 2012-02-02 12:41:35

從豬胰腺Autolysate1四種形式激肽釋放酶的分離和表征
四種形式激肽釋放酶I - IV，主要是從豬胰腺autolysate中分離aminohexyl-賴氨酸- Sepharose和DEAE-瓊脂糖凝膠CL- 6B層析，從10公斤的組織分別獲得65，56，59和41毫克。他們在聚丙烯酰胺凝膠電泳中均質，其他免疫無異，并有相同的氨基酸組成。激肽釋放酶Ⅰ，Ⅱ，Ⅲ包含碳水化合物，但激肽釋放酶IV是基本上無碳水化合物。減少巰基乙醇，他們產生了兩種不同分子量的多肽鏈。激肽釋放酶I和II的H（重型）鏈是一樣的，稱為Hj。它是一種糖蛋白，具有明顯的分子量為21,000，而III和IV鏈，稱為H，H鏈分子量為17000，是缺乏碳水化合物的H鏈。同樣，鏈激肽釋放酶I和III的L（輕）鏈，稱為LL，是糖蛋白，其分子量12000，而從II和IV的L，對應于無碳水化合物L鏈，分子量為8500 。因此，激肽釋放酶I - IV可分別表示為 Hil，HiL，HJLJ，和HLi。他們對合成的底物有比較專一的活性和Km值。激肽釋放酶的I - IV的等電點幾乎是相同的，在4.0-4.1之間。

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2樓2012-02-01 17:56:45