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糾結(jié)死了啊,英譯漢!TT
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Higher percentage of organic solvents gave an incomplete separation with some peaks co-eluting, while higher percentage of water in the mobile phase resulted in peak broadening and prolongation of the analysis. With an injection volume of 30μL, the peaks in the chromatogram were poorly separated, given that a high concentration of analytes can result in broad peaks and co-elution, while 10 μL were in the other hand too low. After that, we did experiments with several different mobile phases composed of either methanol and water or aceto- nitrile and water, where the percentage of the non-polar solvent ranged from 30 to 80%. |

金蟲 (小有名氣)
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高比例的有機(jī)溶劑會(huì)導(dǎo)致多峰共洗脫的不完全分離,在流動(dòng)相中的高比例水會(huì)導(dǎo)致峰加寬以及分析時(shí)間的延長。 當(dāng)加入量為30μL,色譜峰分離較差,假定高濃度的分析物會(huì)導(dǎo)致峰加寬和共洗脫,那么另一方面,10微升的加入量濃度則太低。 之后,我們用含有甲醇和水或乙腈和水的不同的流動(dòng)相進(jìn)行實(shí)驗(yàn),其中非極性溶劑的比例為30%至80%。 |

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