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gl19860312木蟲 (正式寫手)
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[RT-PCR相關(guān)] PCR相關(guān)的專業(yè)網(wǎng)站 已有9人參與
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  一個PCR相關(guān)的專業(yè)網(wǎng)站，內(nèi)容很全 http://www.pcrlinks.com/實驗方法與步驟詳解 http://swjsx.ntmc.edu.cn/Protocol/home.htm 美國AXYgen公司 http://www.axygen.com/NewFiles/pcr.html http://www.alkami.com/ This 158 page PCR manual covers the following topics PCR Primer design PCR Methods PCR Polymerases PCR Variables PCR Troubleshooting Special PCR Topics Appendixes and a comprehensive Index BioGuide - PCR http://bip.weizmann.ac.il/mb/bioguide/pcr/contents.html 魏茨曼基因組和生物信息學研究所的一個網(wǎng)頁，介紹PCR的初步知識。 包括： What is PCR? What are some good reference books for PCR? How should I select a set of primers to use for PCR? Programs for designing PCR primers What is "Hot-start" PCR? What is AP-PCR or RAPD PCR? What is "Touchdown" PCR? Is there a simple method to sequence lambda, M13, or plasmid clones using PCR? A Look at PCR Cloning Tools http://www.dxy.cn/bbs/post/view? ... mp;tpg=63&age=0 PCR常見問題及回答 http://www.invitrogen.com.cn/techtitle/main/faq_PCR.htm#2 PCR和Realtime PCR的原理講的很透, 一個介紹Realtime PCR的教學網(wǎng)站，里面的powerpoint文件非常好 http://www.med.sc.edu:85/pcr/realtime-home.htm SYBR Green PCR http://www.dxy.cn/bbs/post/view? ... =1&age=0#638781 PCR QUICK GUIDE http://www.wzw.tum.de/gene-quantification/quick-guide-pcr.pdf http://www.sfb363.uni-halle.de/m ... ay/sturtevant00.pdf http://www.wooster.edu/biology/courses/bio309/default.html PCR拼接 http://www.dxy.cn/bbs/post/view? ... mp;tpg=47&age=0 知道蛋白如何尋找其氨基酸序列 http://www.dxy.cn/bbs/post/view? ... mp;tpg=36&age=0 http://us.expasy.org/cgi-bin/niceprot.pl?P08253 關(guān)于DNA的flash版和文字版 http://www.pbs.org/wgbh/nova/genome/dna.html# ABI Prism 7000 簡要使用說明 http://www.genomultix.com/tech/7000.htm 好書推薦：Practical Protocols of Gene Engineering http://www.estarray.org/books/ http://www.life.uiuc.edu/bio100/ ... echclonerecomb.html Website dealing with cloning and molecular analysis of genes http://www.ndsu.nodak.edu/instru ... /cloning/clone3.htm Some really wonderful illustrations of the techniques of genetic engineering! http://www.uic.edu/classes/phar/phar331/lecture6/ A truly amazing global resource page on cloning, stem cell research, and related ethical issues, including recent news articles and legislative updates http://genomics.phrma.org/cloning.html Animation and article on interspecies cloning http://www.brookscole.com/chemis ... speciesCloning.html From PBS...an animation that illustrates how stem cells are cultivated http://www.pbs.org/newshour/health/stem_cells.swf Animations illustrating the use of viruses as vectors for gene therapy http://www.technologyreview.com/articles/5940.htm Animations of techniques used for cloning mammals, as well as a create-your-own-clone link http://library.thinkquest.org/24355/data/details/media/index.html Another animation of PCR http://www.people.virginia.edu/~rjh9u/pcranim.html Animations of restriction digests and gel electrophoresis http://www.biology.ucsc.edu/classes/bio20L/animate Gel electrophoresis techniques and simulations A wonderful website that includes links to a virtual lab on agarose gel electrophoresis: http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/ A Massachusetts Institute of Technology website dealing with Southerns, Northerns, Westerns, and cloning: http://web.mit.edu/esgbio/www/rdna/rdna.html 2-D gel electrophoresis of proteins: http://www.fitzscipress.com/Chap05.pdf A website containing lots of links to electrophoresis techniques and simulations: http://biobenchelper.hypermart.net/pr/electrophoresis.htm Want to learn more about chemical bonds? Check out this website: http://hyperphysics.phy-astr.gsu.edu/hbase/chemical/bond.html 多個錄象-多個著名公司 http://www.biocompare.com/video.asp PCR和 RFLP動畫片 http://medtech.cls.msu.edu/ISL/immunology/pcr.htm http://medtech.cls.msu.edu/ISL/immunology/rflp.htm redasoft visual cloning 2000 patch http://www.dxy.cn/bbs/post/view? ... mp;tpg=26&age=0 分子生物學屏幕保護程序下載 pcr http://biosoft.biosino.org/down/PCR.exe 蛋白 http://biosoft.biosion.org/down/ssaver.zip amavi100 http://ourworld.compuserve.com/homepages/esmsoftware/amavi100.zip dna旋轉(zhuǎn) http://www.rocketdownload.com/dnload/dna.exe ddnabase http://www.rocketdownload.com/dnload/ddnabase.exe cyclovir http://mama.indstate.edu/pfaffle/software/cyclovir.exe http://biosoft.biosino.org/down/source.exe http://ftp.monash.edu.au//pub/win.galttech/gtmolecules.zip 分子生物學相關(guān)軟件 http://gene.tmmu.com.cn/biosoft/content.html １.DNA分析：數(shù)據(jù)庫GenBank, EMBL, DDBJ 查詢軟件；用來幫助進行PCR引物設計與基因探針設計的軟件；功能很全面的DNA序列分析工具包；DNA分析與繪圖軟件,可繪制線性或環(huán)形DNA圖；限制酶消化工具；以Java語言寫成的序列比較查看器２.RNA分析：RNA二級結(jié)構(gòu)分析軟件；預測RNA二級結(jié)構(gòu)圖３.蛋白質(zhì)分析：蛋白序列分析軟件包；研究膜蛋白拓撲學結(jié)構(gòu)和其它特性的JAVA軟件４.生化教學：生化基礎概念演示教學程序； 生化教學文件 ５.生化工程：生物反應器（發(fā)酵罐）設計軟件；發(fā)酵實時模擬軟件；計算機輔助設計特別是生物工藝設計的軟件包６.序列格式轉(zhuǎn)換：FASTA與BLAST查詢輸出文件的處理軟件７.引物分析８.序列綜合分析９.檢索與閱讀１０.基因芯片 |
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