FIG. 1. Schematic presentation of the single specific primer PCR used to clone the csp gene. A 1-kb partial fragment of the csp gene was amplified from C. sordellii NCIB10717 total DNA with KAG209 and KAG210 PCR primers, designed in highly conserved regions among Cbp, Cnp, and Cpa. Using a HindIII DNA library of C. sordellii NCIB10717 as a template, PCR was conducted with mF2, located on pKF3, and with KAG212 or KAG213, located on the known 1-kb fragment. Nucleotide sequences of the upstream, the known 1-kb, and the downstream DNA fragments showed an open reading frame of Csp. The thick arrowed line depicts the csp gene. The filled and open areas indicate nucleotide sequence-determined (known) and nondetermined (unknown) regions, respectively.
上述做了標(biāo)記的部分看不懂~~什么是HindIII DNA庫，mF2位于質(zhì)粒pKF3上面，和PCR有什么關(guān)系~~
求解釋~~
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