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huangminghe金蟲 (初入文壇)
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[求助]
關(guān)于利用旋轉(zhuǎn)圓盤電極測量動力學(xué)參數(shù)問題
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我利用旋轉(zhuǎn)圓盤電極研究P450酶對藥物的催化,我首先固定藥物的濃度,測量了不同轉(zhuǎn)速下的催化電流,得到了J−1 vs ω−1/2 的線性關(guān)系,我想利用 Koutecky− Levich equations 計算 the number of electrons transferred (n) and J K can be obtained from the slope and intercept of the Koutecky−Levich plots。但是這個公式中的擴散系數(shù)和運動粘度我不知道是多少,有沒有人知道這些參數(shù)我應(yīng)該怎么查到或者通過什么計算方法可以得到,我用的電解質(zhì)是0.1M pH=7.4 的磷酸鹽緩沖溶液,藥物選擇的是硝苯地平,酶是CYP3A4。請理解的給我一些建議,謝謝! |

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