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Analysis. Aliquots of saturated sodium 4-nitrobenzenesulphonate solutions were transferred into a tarred volumetric flask. The concentration of sodium 4-nitrobenzenesulphonate in aqueous solutions was determined using a Shimadzu-6A high-performance liquid phase chromatograph (HPLC). The chromatographic column used was a unimicro Kromasil C18, 5 (250mm × 4.6mm) maintained at 308.2 K. The HPLC system consisted of a Shimadzu SPD-6A UV single wavelength spectrophotometric detector was set to 270 nm. The mobile phase consists of three eluents which are water, Na2SO4 and H3PO4. The concentration ranges from (0 to 1.5) mgmL-1 of analytes were used for construction of calibration curves. Each analysis was repeated three times, and the average value of the three measurements was considered as the final value of the analysis. The uncertainty of the experimental solubility values is about 2.0 %. The uncertainty in the solubility can be due to uncertainties in the temperature measurements and weighing procedure. |
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至尊木蟲 (職業(yè)作家)
- 翻譯EPI: 110
- 應(yīng)助: 13 (小學(xué)生)
- 貴賓: 0.566
- 金幣: 17176.4
- 散金: 1586
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- 帖子: 3337
- 在線: 545.1小時(shí)
- 蟲號(hào): 1238300
- 注冊(cè): 2011-03-19
- 性別: GG
- 專業(yè): 金屬有機(jī)化學(xué)
【答案】應(yīng)助回帖
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-軒影鳶: 金幣+12, 翻譯EPI+1, ★★★很有幫助 2012-04-24 14:30:05
-軒影鳶: 金幣+12, 翻譯EPI+1, ★★★很有幫助 2012-04-24 14:30:05
| 分析。4－硝基苯磺酸鈉的飽合水溶液轉(zhuǎn)移到定容燒瓶中。用HPLC檢測(cè)4－硝基苯磺酸鈉在水溶液中的濃度。用C18色譜柱，維持溫度在308.2K。HPLC系統(tǒng)由SHIMADZU SPD-6A組成， 紫外單一波長(zhǎng)設(shè)定在270nm處。流動(dòng)相由水，Na2SO4和H3PO4組成。濃度從0到1.5mg/ml分別進(jìn)行分析，制成曲線。每一個(gè)分析重復(fù)三次，平均值作為最后分析值。實(shí)驗(yàn)誤差大約在2%左右。實(shí)驗(yàn)的不確定性主要由實(shí)驗(yàn)室溫度和稱量引起。 |
2樓2012-04-24 14:21:10
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