liangxingqiu

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請(qǐng)問(wèn)蟲(chóng)子們：你們有沒(méi)有做過(guò)油脂里的總酚含量測(cè)定？我們現(xiàn)在測(cè)遇到了一些問(wèn)題，很無(wú)助，周?chē)紱](méi)有人在做，不知道該怎么繼續(xù)了？

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1樓 2012-04-25 15:10:53

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sesame_oil

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http://www.instrument.com.cn/download/shtml/179958.shtml

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2樓2012-04-25 15:21:02

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tangbohejin

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沒(méi)找到

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3樓2012-04-25 15:37:24

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liangxingqiu

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引用回帖:

2樓: Originally posted by sesame_oil at 2012-04-25 15:21:02:
http://www.instrument.com.cn/download/shtml/179958.shtml

不是生育酚，是總酚

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4樓2012-04-25 15:42:23

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qiaqia832

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請(qǐng)問(wèn)你用的什么方法，福林酚法測(cè)的嗎，出現(xiàn)什么問(wèn)題了？個(gè)人覺(jué)得要測(cè)的話(huà)你得先把總酚提取出來(lái)再測(cè)，我只測(cè)過(guò)果汁的，用的是福林酚法和高效液相

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5樓2012-04-25 15:49:12

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liangxingqiu

金蟲(chóng) (正式寫(xiě)手)

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引用回帖:

5樓: Originally posted by qiaqia832 at 2012-04-25 15:49:12:
請(qǐng)問(wèn)你用的什么方法，福林酚法測(cè)的嗎，出現(xiàn)什么問(wèn)題了？個(gè)人覺(jué)得要測(cè)的話(huà)你得先把總酚提取出來(lái)再測(cè)，我只測(cè)過(guò)果汁的，用的是福林酚法和高效液相

是的，福林酚法，我們是測(cè)油的總酚含量，先用正己烷和甲醇提取出來(lái)，再測(cè)，按照文獻(xiàn)上的操作，但是吸光度卻很低，假若增加樣品溶液的量又會(huì)產(chǎn)生沉淀。Briefly for the extraction of phenolic compounds,
10 g of oil was mixed with hexane (50 ml). This mixture
was placed into a separation funnel and 20 ml of methanol:
water (60:40, w:w) was added. The mixture was
shaken for 2 min and the separated water phase was
removed. This procedure was applied three times and all
the water phases were combined and used for TP analysis.
For TP analysis, the mixture of the sample solution
(50 ll), distilled water (3 ml), 250 ll Folin–Ciocalteu’s
reagent solution, and 7% Na2CO3 (750 ll) was vortexed
and incubated for 8 min at room temperature. Then
950 ll of distilled water was added. The mixture was
allowed to stand for 2 h at room temperature. The
absorbance was measured at 765 nm against distilled
water as blank

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6樓2012-04-25 17:38:58

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user59888

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liangxingqiu(金幣+1): 謝謝參與

可以去西北農(nóng)林的食品學(xué)院看看，有這個(gè)專(zhuān)業(yè)

[ 發(fā)自手機(jī)版 http://www.gaoyang168.com/3g ]

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7樓2012-04-25 18:15:37

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myselfsky8159

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遇到什么問(wèn)題了？

贊一下

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8樓2012-04-25 20:37:07

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liangxingqiu

金蟲(chóng) (正式寫(xiě)手)

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引用回帖:

8樓: Originally posted by myselfsky8159 at 2012-04-25 20:37:07:
遇到什么問(wèn)題了？

我們是測(cè)油的總酚含量，先用正己烷和甲醇提取出來(lái)，再測(cè)，按照文獻(xiàn)上的操作，但是吸光度卻很低，才0.08左右，代到標(biāo)曲里面算到的總酚含量也很低，明顯錯(cuò)的。假若增加樣品溶液的量又會(huì)產(chǎn)生沉淀。方法是：Briefly for the extraction of phenolic compounds,
10 g of oil was mixed with hexane (50 ml). This mixture
was placed into a separation funnel and 20 ml of methanol:
water (60:40, w:w) was added. The mixture was
shaken for 2 min and the separated water phase was
removed. This procedure was applied three times and all
the water phases were combined and used for TP analysis.
For TP analysis, the mixture of the sample solution
(50 ll), distilled water (3 ml), 250 ll Folin–Ciocalteu’s
reagent solution, and 7% Na2CO3 (750 ll) was vortexed
and incubated for 8 min at room temperature. Then
950 ll of distilled water was added. The mixture was
allowed to stand for 2 h at room temperature. The
absorbance was measured at 765 nm against distilled
water as blank

贊一下

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9樓2012-04-25 22:48:24

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majiaona

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好像有國(guó)標(biāo)的吧

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10樓2012-04-25 23:26:11

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qiaqia832

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引用回帖:

6樓: Originally posted by liangxingqiu at 2012-04-25 17:38:58:
是的，福林酚法，我們是測(cè)油的總酚含量，先用正己烷和甲醇提取出來(lái)，再測(cè)，按照文獻(xiàn)上的操作，但是吸光度卻很低，假若增加樣品溶液的量又會(huì)產(chǎn)生沉淀。Briefly for the extraction of phenolic compounds,
10 g ...

吸光值低，可能是總酚含量太低，可以考慮濃縮一下提取液，用旋轉(zhuǎn)蒸發(fā)濃縮，或者真空干燥，這樣試試看。要么就換個(gè)提取方法。

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11樓2012-04-26 08:20:54

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threestone5868

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國(guó)標(biāo)啊

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12樓2012-04-27 22:35:27

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