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lina8804新蟲 (初入文壇)
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論文翻譯求助，大家?guī)蛶兔Γx謝
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3.8 試劑加入順序?qū)w系熒光的影響 固定其它條件，考察試劑加入順序?qū)CDO-DNA體系熒光猝滅的影響，結(jié)果如圖3.21和3.22所示。 根據(jù)圖3.21和3.22測(cè)定的熒光強(qiáng)度作BCDO-DNA體系熒光猝滅強(qiáng)度ΔF與試劑加入順序的變化曲線。結(jié)果如圖3.23所示。本實(shí)驗(yàn)中試劑的不同的加入順序?qū)CDO與DNA體系的影響都很大。但緩沖溶液在最后加入時(shí)，BCDO-DNA體系的熒光猝滅值ΔF最大。因此采用緩沖溶液最后加入。 由以上實(shí)驗(yàn)條件的選擇得到測(cè)定BCDO-DNA體系的較佳實(shí)驗(yàn)條件，DNA的濃度為5.0mg•L-1，BCDO的濃度為3.0×10-5mol•L-1，pH=6.8時(shí)，反應(yīng)時(shí)間為5min，溶劑體積小于10%，溶液溫度為室溫，加入順序?yàn)锽CDO+緩沖溶液+DNA。根據(jù)較佳實(shí)驗(yàn)條件，對(duì)BCDO-DNA體系的干擾試驗(yàn)，工作曲線，作用方式，進(jìn)行了研究，并通過EB法作對(duì)照，對(duì)BCDO-DNA體系的猝滅方式進(jìn)行了進(jìn)一步的判斷。 |
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fengchunyun
木蟲 (小有名氣)
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【答案】應(yīng)助回帖
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愛與雨下: 金幣+2 2012-04-27 15:01:14
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lina8804: 金幣+25, 翻譯EPI+1, ★★★很有幫助, 非常感謝啊！ 2012-05-02 10:43:42
愛與雨下: 金幣+2 2012-04-27 15:01:14
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lina8804: 金幣+25, 翻譯EPI+1, ★★★很有幫助, 非常感謝啊！ 2012-05-02 10:43:42
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3.8 The effect of the addition order of reagents on the fluorescence of the system Other factors were fixed, the effect of the addition order of reagents on the fluorescence quenching of the BCDO-DNA system were investigated, the results were shown in Figure 3.21 and 3.22. According to the fluorescence intensity investigated in Figure 3.21 and 3.22, the curve was made of the strength of fluorescence quenching (ΔF) of the BCDO-DNA system and the addition order of reagents, as was shown in Figure 3.23. In this experiment, the different addition order of reagents had a great influence on the system both BCDO and DNA. However, when the buffer solution was added at last, the maximum fluorescence quenching value of the BCDO-DNA system was obtained. So, the buffer solution should be added finally. From the selection of above experiment condition, the better experimental factors determining the BCDO-DNA system were made, the concentration of DNA and BCDO were 5.0mg•L-1 and 3.0×10-5mol•L-1, respectively; pH was 6.8, the reaction time was 5min, the volume of solvent was less than 10%, the temperature of the solution was room temperature(20℃), the addition order was BCDO + buffer solution + DNA. According to the better experimental condition, studies of the interference test, working curve, action mode of the BCDO-DNA system were made, compared with the EB method, the quenching method of the BCDO-DNA system was further determined. 翻譯的不好，呵呵，拋磚引玉哈~~~ |
2樓2012-04-27 14:56:38
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