24小時熱門版塊排行榜

北京石油化工學(xué)院2026年研究生招生接收調(diào)劑公告

返回列表

XUMIN6891

金蟲 (初入文壇)

應(yīng)助: 0 (幼兒園)
金幣: 1015.5
帖子: 41
在線: 19.1小時
蟲號: 1127506
注冊: 2010-10-20
性別: MM
專業(yè): 獸醫(yī)傳染病學(xué)

[求助] 把這段中文翻譯成英語

摘要：目的：構(gòu)建抗菌肽LNK-16基因6拷貝，12拷貝串聯(lián)體，并將目的基因串聯(lián)產(chǎn)物構(gòu)建到表達(dá)載體pET30a(+)上。方法：以實驗室構(gòu)建好的LNK-16基因3拷貝的重組質(zhì)粒為模板設(shè)計引物，通過PCR擴(kuò)增合成2段3拷貝的LNK-16片段，分別命名為G1，G2。其中片段G1的上下游分別設(shè)有Nco I和Xho I酶切位點，片段G2的上下游分別設(shè)有EcoR I和Nco I酶切位點，分別把G1和G2雙酶切回收，然后將pET30a(+)載體用EcoR I ，Xho I雙酶切回收，最后在一個體系里將片段G1，G2，和pET30a(+)載體連接。這樣得到含6拷貝的LNK-16基因。然后再以它為模板設(shè)計引物，通過PCR擴(kuò)增合成2段6拷貝的LNK-16片段分別命名為G3，G4。其中G3的上下游分別設(shè)有EcoR I和Hind III酶切位點，G4的上下游分別設(shè)有Hind III和Xho I酶切位點，分別把G3和G4雙酶切回收，然后將G3，G4和經(jīng)過EcoR I 、Xho I雙酶切回收的pET30a(+)載體連接，這樣就得到含12拷貝的LNK-16基因。結(jié)果：PCR鑒定、雙酶切鑒定和DNA測序證明6拷貝，12拷貝基因的重組質(zhì)粒構(gòu)建成功。結(jié)論：該方法能方便高效地獲得所需要的多拷貝基因，為進(jìn)一步克隆到表達(dá)載體進(jìn)行高效表達(dá)打下基礎(chǔ)。

回復(fù)此樓

» 猜你喜歡

材料考研調(diào)劑已經(jīng)有3人回復(fù)
材料調(diào)劑已經(jīng)有12人回復(fù)
英一數(shù)一408，總分284，二戰(zhàn)真誠求調(diào)劑已經(jīng)有14人回復(fù)
085410 一志愿211 22408分?jǐn)?shù)359求調(diào)劑已經(jīng)有4人回復(fù)
271求調(diào)劑已經(jīng)有19人回復(fù)
385分生物學(xué)（071000）求調(diào)劑已經(jīng)有3人回復(fù)
一志愿安徽大學(xué)計算機(jī)科學(xué)與技術(shù)學(xué)碩，331分求調(diào)劑已經(jīng)有3人回復(fù)
318求調(diào)劑，計算材料方向已經(jīng)有8人回復(fù)
291求調(diào)劑已經(jīng)有25人回復(fù)
一志愿北京科技大學(xué)085601材料工程英一數(shù)二初試總分335求調(diào)劑已經(jīng)有6人回復(fù)

1樓 2012-05-24 10:25:56

已閱關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

AnnF

版主 (文壇精英)

翻譯EPI: 6
應(yīng)助: 197 (高中生)
貴賓: 0.881
金幣: 443227
散金: 5581
紅花: 2315
沙發(fā): 3
帖子: 27689
在線: 3136.6小時
蟲號: 1223787
注冊: 2011-03-07
專業(yè): 藥物化學(xué)
管轄: 醫(yī)藥類考試

★ ★
XUMIN6891: 金幣+2, 翻譯EPI+1, 你用機(jī)器直譯的把？ 2012-05-24 17:27:36
愛與雨下: 翻譯EPI-1 2012-05-24 21:39:54

Abstract: Objective: To build the antimicrobial peptide LNK-16 gene 6 copy, 12 copies of the tandem body, and the target gene series products built into the expression vector pET30a (). The method: to build a good laboratory LNK-16 gene copies of the recombinant plasmid as a template design primers synthesized by PCR amplification of paragraph 2 of 3 copies of LNK-16 fragment, were named G1, G2. Fragment G1 of the upstream and downstream, respectively, with Nco I and Xho I sites upstream and downstream of the fragment of G2 with EcoR I and Nco I restriction sites, respectively, G1 and G2, double digestion recovery, and pET30a () vector using the EcoR I and Xho I double digestion recovery, the last in a system where the fragments G1, G2, and and pET30a () vector. This LNK-16 containing six copies of the gene. LNK-16 fragments and then using it as the template design primers synthesized by PCR amplification of paragraph 2 of 6 copies were named as G3, G4, Which the G3 upstream and downstream, respectively, with EcoR I and Hind III restriction sites, the G4 upstream and downstream, respectively, with Hind III and Xho I sites, respectively, G3 and G4 double digestion recovery, then to G3, G4, after the EcoR I, Xho I double digestion recovery of pET30a () vector, so that the LNK-16 gene containing 12 copies. Results: PCR identification of restriction enzyme digestion and DNA sequencing to prove that six copies of the 12 copies of recombinant plasmid was successfully constructed. Conclusion: This method can be easily and efficiently obtain the required multi-copy gene for efficient expression lay the foundation for further cloned into the expression vector.

贊一下

回復(fù)此樓

2樓2012-05-24 11:04:53