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[求助]
請(qǐng)高手幫忙翻譯～～依據(jù)這部分做實(shí)驗(yàn) 心情急切 謝謝謝謝��！
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MATERIALS AND METHODS Female ICR mice (Harlan, Houston, Tex.) weighing 18-23 g were ran- domly divided into 3 groups of 20 and given powdered feed (Purina Lab Chow 5002) supplemented with 0, 0.125, and 0.5% EQ HCI, respectively. Mice were killed by cervical dislocation, 4/group, after 2, 4, 6, and 10 wk of feeding; the liver, kidney, lung, and brain tissues were rapidly excised and weighed. Approximately 200 mg of each tissue sample with the ex- ception of the lung tissue was homogenized in 10 volumes (w/v) of ice- cold acetonitrile-water (7 : 3, v/v) with a Polytron (Brinckmann), then cen- trifuged for 10 min at 5000 x g. The clear supernatant was transferred into a screw-capped glass vial, centrifuged, then stored in a freezer (-20°C) for 2-3 h or until the two layers separated. Due to the small size of the lung tissue, most of it was homogenized in 20 volumes of solvent. The clear upper layers, approximately 60% of the total volume, contained most (>90%) of the EQ; therefore, only the upper layers were analyzed by HPLC detected by the fluoromonitor. In order to avoid possible diur- nal fluctuations, mice were killed at 8:00-10:00. Approximately 200 mg of another liver sample from each mouse was homogenized in 10 volumes (w/v) of ice-cold 0.25 M perchloric acid and centrifuged for 10 min at 5000 x g for the determination of GSH level. Three groups of mice, 4/group, were killed after 14 wk of feeding, 1/group/d from each feeding group, and the mitochondria were isolated from the liver, saving 2 samples of approximately 200 mg each to deter- mine the EQ residue and GSH levels. The liver mitochondria were iso- lated in 2.5 M sucrose buffer by differential centrifugaron Gohnson and Lardy, 1967) and the protein concentration was determined by the biuret method (Gornall et al., 1949). |
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sesame_oil
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愛(ài)與雨下: , ？ 2012-06-03 21:05:01
zl0921516: 金幣+5, ★★★很有幫助 2012-06-04 10:07:08
愛(ài)與雨下: , ？ 2012-06-03 21:05:01
zl0921516: 金幣+5, ★★★很有幫助 2012-06-04 10:07:08
| 雌性ICR小鼠（哈倫，休斯敦，得克薩斯州），體重18-23克然進(jìn)行隨機(jī)分為3組，每組20和粉狀飼料（普瑞納實(shí)驗(yàn)室周5002）輔以0，0.125，0.5％EQ人機(jī)交互，分別。頸椎脫位，4/group，后2，4，6，和10周處死小鼠，喂養(yǎng);肝，腎，肺，腦組織迅速切除并稱重。大約200毫克的每個(gè)組織樣本前肺組織勻漿10卷（W / V）冰ception冷乙腈 - 水（7：3，V / V），然后與寶創(chuàng)（Brinckmann）中心trifuged為5000 XĞ10分鐘。清澈的上清液轉(zhuǎn)移到一個(gè)螺絲帽的玻璃瓶，離心，然后存放在冰箱（-20℃）為2-3小時(shí)，或直至兩層分離。由于規(guī)模小肺組織，其中大部分被勻漿溶劑，在20卷。明確的上層，總量約60％，載大部分的EQ（> 90％），因此，只有上層進(jìn)行了分析高效液相色譜法檢測(cè)由fluoromonitor。為了避免可能diur信號(hào)波動(dòng)，處死小鼠在8:00-10:00。約200毫克。另外從各組小鼠肝勻漿樣品10卷冰冷的0.25 M過(guò)氯酸（W / V），離心10分鐘G為5000 X GSH水平的決心。 三組小鼠，4/group，喂養(yǎng)14周后處死，1/group/d每次喂食組，線粒體分離從肝，節(jié)省約200毫克，每2個(gè)樣品阻止挖掘情商殘留和GSH水平。肝線粒體是ISO-lated差centrifugaron Gohnson和2.5 M蔗糖緩沖拉迪，1967年）和蛋白質(zhì)濃度的縮二脲的測(cè)定方法（Gornall等，1949）。撤消修改Cíxìng ICR xiǎo shǔ (hā lún, xiūsīdūn, dekèsàsī zhōu), tǐzhòng 18-23 kè rán Jìnxíng suíjī fēn wéi 3 zǔ, měi zǔ 20 hé fěn zhuàng sìliào (pǔ ruì nà shíyàn shì Zhōu 5002) fǔ yǐ 0,0.125, 0.5% EQ rén jī jiāohù, fēnbié. Jǐngchuí tuōwèi, 4/group, hòu 2,4,6, hé 10 zhōu chǔsǐ xiǎo shǔ, Wèiyǎng; gān, shèn, fèi, nǎo zǔzhī xùnsù qiēchú Bìng chēng zhòng. Dàyuē 200 háokè de měi gè zǔzhī yàngběn qián Fèi zǔzhī yún jiāng 10 juǎn (W / V) bīng ception Lěng yǐjīng - shuǐ (7: 3, V/ V), ránhòu yǔ bǎo chuàng (Brinckmann) zhōngxīn Trifuged wèi 5000 X Ğ 10 fēnzhōng. Qīngchè de shàng qīng yè zhuǎnyí Dào yīgè luósī mào de bōlí píng, líxīn, ránhòu cúnfàng zài bīngxiāng ( -20℃) Wèi 2-3 xiǎoshí, huò zhízhì liǎng céng fēnlí. Yóuyú guīmó xiǎo Fèi zǔzhī, qízhōng dà bùfèn bèi yún jiāng róngjì, zài 20 juǎn. Míngquè de shàngcéng, zǒng liàng yuē 60% , zài Dà bùfèn de EQ (> 90% ), yīncǐ, zhǐyǒu shàngcéng jìnxíngle fēnxī Gāoxiào yè xiāng sèpǔ fǎ jiǎncè yóu fluoromonitor. Wèile bìmiǎn kěnéng diur Xìnhào bōdòng, chǔsǐ xiǎo shǔ zài 8:00-10:00. Yuē 200 háokè. Lìngwài cóng gè zǔ xiǎo shǔ gān yún jiāng yàngpǐn 10 juǎn Bīnglěng de 0.25 Mguò lǜ suān (W / V), líxīn 10 fēnzhōng G wèi 5000 X GSH shuǐpíng de juéxīn. Sān zǔ xiǎo shǔ, 4/group, wèiyǎng 14 zhōu hòu chǔsǐ, 1/Group/d měi cì wèishí zǔ, xiànlìtǐ fēnlí Cóng gān, jiéshěng yuē 200 háokè, měi 2 gè yàngpǐn zǔzhǐ Wājué qíngshāng cánliú hé GSH shuǐpíng. Gān xiànlìtǐ shì ISO- Lated chà centrifugaron Gohnson hé 2.5 M zhètáng huǎnchōng Lā dí,1967 nián) hé dànbáizhí nóngdù de suō èr niào de cèdìng Fāngfǎ (Gornall děng, 1949). |
4樓2012-06-03 19:00:16
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愛(ài)與雨下: 金幣+1 2012-06-03 21:04:38
zl0921516: 金幣+15, 翻譯EPI+1, ★★★★★最佳答案 2012-06-04 10:06:58
愛(ài)與雨下: 金幣+1 2012-06-03 21:04:38
zl0921516: 金幣+15, 翻譯EPI+1, ★★★★★最佳答案 2012-06-04 10:06:58
|
雌性ICR鼠（18-23g）隨機(jī)分成三組，每組20只并正常喂養(yǎng)，飼料中分別添加0，0.125和0.5%的EQ HCI，在第2，4，6，10周的時(shí)候分別取每組中的四只老鼠斷頸處死，迅速取出肝，腎，肺，腦組織并稱重。大約200mg的組織（除去肺組織）懸浮于10倍體積預(yù)冷的乙腈-水（7:3體積比），然后5000g離心10min，上清液轉(zhuǎn)移到有蓋子的玻璃小瓶中，儲(chǔ)存在-20°2-3h或者等到兩相分離。由于肺組織比較小，一般懸浮于20倍體積的溶劑，清透的上清液大約占總體積的60%左右，包括EQ（超過(guò)90%），因此，只有上相才有高效液相分析，為了避免日常的波動(dòng)，老鼠仔8點(diǎn)到十點(diǎn)之間處死。大約200mg肝組織懸浮于10倍體積預(yù)冷的0.25M過(guò)氯酸，5000g離心10min以達(dá)到檢測(cè)谷胱甘肽水平。 第14周處死的老鼠，每組中取一只從肝組織分離線粒體，大約200mg來(lái)檢測(cè)EQ和GSH水平，肝線粒體用2.5M蔗糖不同離心力來(lái)分離，用雙縮脲法檢測(cè)蛋白濃度。（太老的文獻(xiàn)了） 大概是這樣吧！ |
3樓2012-06-03 18:01:46
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