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jyalhc銅蟲 (小有名氣)
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[求助]
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題目：Identification of the genus Epimedium with DNA barcodes 期刊名：Journal of Medicinal Plants Research 卷/頁：Vol. 5(28), pp. 6413-6417, 30 November, 2011 |
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leimiao_hit
木蟲之王 (文學(xué)泰斗)
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amisking: 金幣+2, BioEPI+1, 鼓勵(lì)發(fā)帖交流問題。 2012-06-11 20:27:59
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jyalhc: 金幣+8, ★★★★★最佳答案 2012-06-19 23:54:35
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FN Thomson Reuters Web of Knowledge VR 1.0 PT J AU Jiang, Y Ding, CB Zhang, L Yang, RW Zhou, YH Tang, L AF Jiang, Yu Ding, Chun-bang Zhang, Li Yang, Ruiwu Zhou, Yonghong Tang, Li TI Identification of the genus Epimedium with DNA barcodes SO JOURNAL OF MEDICINAL PLANTS RESEARCH LA English DT Article DE Epimedium; DNA barcode; ITS; rbcL; matK; psbA-trnH ID LAND PLANTS; SPECIES IDENTIFICATION; RBCL; SEQUENCES; MARKERS AB The traditional identification for Epimedium is inadequate due to the morphological variation and complicated procedure. DNA barcoding is a new technique for species identification by a standard DNA region. However, there has been no consensus emerged for plant barcoding due to the low rates of evolution in plastid DNA. In this paper, we compared the performance of four candidate DNA regions (psbA-trnH, ITS, rbcL, matK) in ten species of Epimedium. To select a suitable barcode, ranking criteria included: the success efficiency of polymerase chain reaction (PCR) amplification and sequencing; genetic divergences; the barcoding gap. The psbA-trnH represented easy amplification, high level of interspecific divergence and well-separated barcoding gap. The other three loci (ITS, rbcL, matK) was inadequate for application. We concluded that the psbA-trnH region was the better potentially DNA barcode for the genus Epimedium. C1 [Jiang, Yu; Ding, Chun-bang; Zhang, Li; Yang, Ruiwu; Tang, Li] Sichuan Agr Univ, Coll Biol & Sci, Yaan 625014, Peoples R China. [Zhou, Yonghong] Sichuan Agr Univ, Key Lab Crop Genet Resources & Improvement, Minist Educ, Yaan 625014, Peoples R China. RP Ding, CB (reprint author), Sichuan Agr Univ, Coll Biol & Sci, Yaan 625014, Peoples R China EM dcb@sicau.edu.cn NR 21 TC 0 Z9 0 PU ACADEMIC JOURNALS PI VICTORIA ISLAND PA P O BOX 5170-00200 NAIROBI, VICTORIA ISLAND, LAGOS 73023, NIGERIA SN 1996-0875 J9 J MED PLANTS RES JI J. Med. Plants Res. PD NOV 30 PY 2011 VL 5 IS 28 BP 6413 EP 6417 DI 10.5897/JMPR11.545 PG 5 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA 873WE UT WOS:000298913200004 ER -------------------------------------------------------------------------------- EF |
4樓2012-06-11 06:42:39
小天龍
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2樓2012-06-11 00:56:40
leimiao_hit
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3樓2012-06-11 06:42:06
xstarsky
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