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| 本帖產(chǎn)生 1 個 翻譯EPI ，點擊這里進行查看 | |||
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[求助]
請幫忙翻譯一段關于生物醫(yī)藥方面的英文，謝謝~
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The RNA thus isolated was quantitatively analyzed and used to synthesize cDNA by RT-PCR in the presence of the reverse transcriptase SuperscriptIII (Invitrogen), with 35 dermal cycles of 94(攝氏度）for 45 sec, 55(攝氏度）for 45 sec and 72(攝氏度） for 1.5 min. Inhibitory effects on gene expression were analyzed on agarose gel. The primers used in the gene amplification were summarized in Table 1. As a control gene, GAPDH was used. [ Last edited by scswpiyly on 2012-6-14 at 16:24 ] |
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8814402
至尊木蟲 (職業(yè)作家)
- 翻譯EPI: 509
- 應助: 18 (小學生)
- 貴賓: 0.381
- 金幣: 12916.1
- 散金: 47
- 紅花: 16
- 帖子: 4183
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- 蟲號: 1184404
- 注冊: 2011-01-06
- 專業(yè): 藥物學其他科學問題
3樓2012-06-14 17:10:57
8814402
至尊木蟲 (職業(yè)作家)
- 翻譯EPI: 509
- 應助: 18 (小學生)
- 貴賓: 0.381
- 金幣: 12916.1
- 散金: 47
- 紅花: 16
- 帖子: 4183
- 在線: 357.8小時
- 蟲號: 1184404
- 注冊: 2011-01-06
- 專業(yè): 藥物學其他科學問題
【答案】應助回帖
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scswpiyly: 金幣+15, 翻譯EPI+1, ★★★★★最佳答案, thanks！ 2012-06-14 17:52:58
scswpiyly: 金幣+15, 翻譯EPI+1, ★★★★★最佳答案, thanks！ 2012-06-14 17:52:58
| The RNA thus isolated was quantitatively analyzed and used to synthesize cDNA by RT-PCR in the presence of the reverse transcriptase Superscript III (Invitrogen), with 35 dermal cycles of 94(攝氏度）for 45 sec, 55(攝氏度）for 45 sec and 72(攝氏度） for 1.5 min. 對由此分離的RNA進行定量分析，并用于通過RT-PCR合成cDNA，在逆轉錄酶Superscript III 存在下，經(jīng)過94攝氏度45秒、55攝氏度45秒和72攝氏度1.5分的35個循環(huán)。Inhibitory effects on gene expression were analyzed on agarose gel. The primers used in the gene amplification were summarized in Table 1. As a control gene, GAPDH was used. |
2樓2012-06-14 17:08:02
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