 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 1 | 1/1 | 返回列表 |
| 查看: 588 | 回復(fù): 0 | ||
snowhurst銀蟲 (小有名氣)
|
[求助]
求潤色
|
|
挖掘并利用作物優(yōu)異抗病基因是進行農(nóng)作物品種遺傳改良的重要途徑。辣椒是我國乃至世界最重要的蔬菜作物之一，黃瓜花葉病毒�。–MV）是目前影響辣椒生產(chǎn)的一種重要病害�；陧椖拷M多年的前期研究，本項目針對栽培辣椒品種中缺乏抗CMV基因的現(xiàn)狀，以高抗CMV辣椒Vc16a為材料，首先采用抗病基因同源序列法和cDNA-AFLP技術(shù)，克隆抗CMV相關(guān)候選RGAs或差異表達基因，其次通過RACE技術(shù)獲得抗CMV相關(guān)候選RGAs或差異表達基因的全長，分析其結(jié)構(gòu)和序列特征，再利用熒光定量PCR技術(shù)分析抗CMV候選基因在不同組織的時空表達特性，最后利用轉(zhuǎn)基因技術(shù)對抗CMV候選基因進行功能驗證，明確其與抗CMV的相關(guān)性，最終克隆出抗CMV基因。項目挖掘的優(yōu)異基因及相關(guān)基礎(chǔ)理論研究結(jié)果對于辣椒及其它作物的抗CMV遺傳育種將具有重要的理論和實踐意義。 Pepper is one of the most cultivated Solanaceae species worldwide. Several disease caused by virus, fungi, bacteria and nematodes can reduce yield and quality of pepper fruits, and cucumber mosaic virus (CMV) is the most important virus disease. Genetic improvement of a variety with a resistance to CMV is slower, most due to the absence of resistance gene of CMV. Capsicum frutescens var. Vc16a with a high resistance to CMV is used in this study. First, CMV resistance gene or differential expression gene is cloned by resistance gene analogue (RGA) and cDNA amplified fragment length polymorphism (cDNA-AFLP). Second, full-length of these candidate gene is obtained by rapid amplification of cDNA ends (RACE), gene structure and sequence features is described, spatial expression characteristics in different tissues is analyzed based on quantitative fluorescent PCR (QF-PCR). Finally, a genetic transformation procedure is obtained for functional validation of candidate genes, if the correlation between the resistance to CMV and candidate genes is significant, resistance gene then is cloned. This approach will support an intensive study of functional analysis of potential resistance genes, and this is particularly well adapted to CMV-resistance-based approaches to breed a new pepper variety. |
回復(fù)此樓» 猜你喜歡
317分 一志愿南理工材料工程 本科湖工大 求調(diào)劑
已經(jīng)有11人回復(fù)
-
332求調(diào)劑
已經(jīng)有12人回復(fù)
-
材料專碩 085600求調(diào)劑
已經(jīng)有5人回復(fù)
-
0703化學(xué)/290求調(diào)劑/本科經(jīng)歷豐富/工科也可
已經(jīng)有14人回復(fù)
-
283求調(diào)劑（080500）
已經(jīng)有14人回復(fù)
-
材料工程專碩求調(diào)劑
已經(jīng)有6人回復(fù)
-
材料專碩調(diào)劑
已經(jīng)有9人回復(fù)
-
求調(diào)劑 有機化學(xué)考研356分
已經(jīng)有11人回復(fù)
-
環(huán)境科學(xué)與工程334分求調(diào)劑
已經(jīng)有8人回復(fù)
-
考研調(diào)劑
已經(jīng)有5人回復(fù)
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 snowhurst 的主題更新
| 1 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 310求調(diào)劑 +9 | 爭取九點睡 2026-03-30 | 9/450 |
|
|---|---|---|---|---|
|
[考研] 求調(diào)劑，一志愿 南京航空航天大學(xué)大學(xué) ，080500材料科學(xué)與工程學(xué)碩 +6 | @taotao 2026-03-26 | 7/350 |
|
|
|
[考研] 375求調(diào)劑 +6 | 雨夏整夜 2026-03-29 | 6/300 |
|
|
|
[考研] 085404求調(diào)劑，總分309，本科經(jīng)歷較為豐富 +6 | 來財aa 2026-03-25 | 6/300 |
|
|
|
[考研] 085600 286分 材料求調(diào)劑 +10 | 麻辣魷魚 2026-03-27 | 11/550 |
|
|
|
[考研] 307求調(diào)劑 +9 | 超級伊昂大王 2026-03-24 | 10/500 |
|
|
|
[考研] 22408 359分調(diào)劑 +4 | Qshers 2026-03-27 | 6/300 |
|
|
|
[考研] 材料學(xué)碩333求調(diào)劑 +11 | 北道巷 2026-03-24 | 11/550 |
|
|
|
[考研] 0856求調(diào)劑 +13 | zhn03 2026-03-25 | 14/700 |
|
|
|
[考研] 329求調(diào)劑 +7 | 星野? 2026-03-26 | 7/350 |
|
|
|
[考研] 一志愿太原理工安全工程300分，求調(diào)劑 +5 | 0857求調(diào)劑. 2026-03-24 | 6/300 |
|
|
|
[考研] 學(xué)碩274求調(diào)劑 +9 | Li李魚 2026-03-26 | 9/450 |
|
|
|
[考研] 322求調(diào)劑 +7 | 宋明欣 2026-03-27 | 7/350 |
|
|
|
[考研] 材料與化工（0856）304求B區(qū)調(diào)劑 +8 | 邱gl 2026-03-27 | 8/400 |
|
|
|
[考研] 考研調(diào)劑 +4 | Sanmu-124 2026-03-26 | 4/200 |
|
|
|
[考研] 085600，材料與化工321分調(diào)劑 +4 | 大饞小子 2026-03-27 | 6/300 |
|
|
|
[考研] 考研調(diào)劑 +9 | 小蠟新筆 2026-03-26 | 9/450 |
|
|
|
[考研] 321求調(diào)劑 +6 | wasdssaa 2026-03-26 | 6/300 |
|
|
|
[考研] 一志愿南航 335分 | 0856材料化工 | GPA 4.07 | 有科研經(jīng)歷 +6 | cccchenso 2026-03-23 | 6/300 |
|
|
|
[考研] 0854人工智能方向招收調(diào)劑 +4 | 章小魚567 2026-03-24 | 4/200 |
|