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揮汗如雨至尊木蟲 (職業(yè)作家)
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[交流]
FASP 酶解
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FASP 酶解: In-gel digestion for mass spectrometry-based proteomics is extremely robust whereas in-solution digestion is more easily automated and minimizes sample handing. Filter aided sample preparation (FASP，濾紙收集樣品), combines theses advantages by completely solubilizing the proteome in sodium dodecyl sulfate (SDS), which is exchanged by urea on a standard filtration device. Peptides after digestion on the filter are pure, allowing single run analysis of orgabelles and unprecedented depth of proteome coverage. http://wenku.baidu.com/view/7599858ccc22bcd126ff0c73.html |
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