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| Addition of the amino acids threonine,serine,proline,and arginine to fermentations of the fungus Glarea lozoyensis influenced both the pneumocandin titer and the spectrum of analogues produced. Addition of threonine or serine altered the levels of the “serine analogues” of pneumocandin B0 and B5 and allowed for their isolation and identification. Proline supplementation resulted in a dose-dependent increase in the levels of pneumocandin B0 and E0, whereas pneumocandin C0 and D0 decreased as a function of proline level. Moreover, proline supplementation resulted in an overall increase in the synthesis of both trans-3- and trans-4-hydroxyproline while maintaining a low trans-4-hydroxyproline to trans-3-hydroxyproline ratio compared to the unsupplemented culture. pneumocandin production and the sythesis of hydroxyprolines was also affected by addition of the proline-related amino acid arginine but not by the addition of glutamine or ornithine.Zinc,cobalt,copper,and nickel,trace elements that are known to inhibit α-ketoglutarate-dependent dioxygenases, affected the pneumocandin B0 titer and altered the levels of pneumocandins B1, B2，B5，B6，and E0，analogues that possess altered proline,ornithine,and tyrosine hydroxylation patterns. |
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Addition of the amino acids threonine,serine,proline,and arginine to fermentations of the fungus Glarea lozoyensis influenced both the pneumocandin titer and the spectrum of analogues produced. 在真菌Glarea lozoyensis（菌種，專業(yè)名詞）中添加蘇氨酸、絲氨酸、脯氨酸和精氨酸會影響pneumocandin（專業(yè)名詞，是卡泊芬凈前體）滴度和產(chǎn)生的類似物光譜。 Addition of threonine or serine altered the levels of the “serine analogues” of pneumocandin B0 and B5 and allowed for their isolation and identification. 添加蘇氨酸和絲氨酸改變pneumocandinB0和B5的“絲氨酸類似物”的水平并影響分離和鑒別。 Proline supplementation resulted in a dose-dependent increase in the levels of pneumocandin B0 and E0, whereas pneumocandin C0 and D0 decreased as a function of proline level. 添加補(bǔ)充脯氨酸導(dǎo)致pneumocandinB0和E0水平隨脯氨酸劑量增加而增加，然而pneumocandinC0和D0降低。 Moreover, proline supplementation resulted in an overall increase in the synthesis of both trans-3- and trans-4-hydroxyproline while maintaining a low trans-4-hydroxyproline to trans-3-hydroxyproline ratio compared to the unsupplemented culture. 除此之外，脯氨酸添加導(dǎo)致反式3和反式4羥基脯氨酸合成整體增加，然而卻和未添加脯氨酸培養(yǎng)相比，添加脯氨酸的反-4、反-3 羥基脯氨酸卻維持在低水平。 pneumocandin production and the sythesis of hydroxyprolines was also affected by addition of the proline-related amino acid arginine but not by the addition of glutamine or ornithine. 脯氨酸相關(guān)氨基酸精氨酸同樣會影響pneumocandin的產(chǎn)物及羥基脯氨酸的合成，而谷氨酸鹽和鳥氨酸則不會。 Zinc,cobalt,copper,and nickel,trace elements that are known to inhibit α-ketoglutarate-dependent dioxygenases, affected the pneumocandin B0 titer and altered the levels of pneumocandins B1, B2，B5，B6，and E0，analogues that possess altered proline,ornithine,and tyrosine hydroxylation patterns. 已知鋅、鈷、銅和錫，痕量元素會抑制α-酮戊二酸依賴性的加雙氧酶，影響 pneumocandin B0滴度，改變 pneumocandin B1，B2，B5，B6和E0，擁有改變脯氨酸、鳥氨酸酪氨酸羥基化模式的類似物的含量。 專業(yè)名詞我沒有翻譯，畢竟不是這個領(lǐng)域的�？吹揭黄形奈墨I(xiàn)，樓主自己參考把。 https://www.cnki.com.cn/Article/CJFDTotal-HBHG201109026.htm 希望都樓主有用。 |
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