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小木蟲論壇-學術科研互動平臺 » 學術交流區(qū) » 論文投稿 » 其他 » 求助論文檢索號
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求助以下一篇論文的檢索號： Author: Xiao-Ying Yin,Yong-Ming Luo,Jian-Jiang Fu,You-Quan Zhong, Qing-Shan Liu. Title: Determination of hyperoside andisoquercitrin in rat plasma by membrane protected micro-solid-phase extraction with high-performance liquid chromatography. Journal: Journal of Separation Science , 2012, 35, 384-391. |
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xxxice
木蟲 (著名寫手)
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Accession number: 20120414718143 Title: Determination of hyperoside and isoquercitrin in rat plasma by membrane-protected micro-solid-phase extraction with high-performance liquid chromatography Authors: Yin, Xiao-Ying1 ; Luo, Yong-Ming1 ; Fu, Jian-Jiang1 ; Zhong, You-Quan1 ; Liu, Qing-Shan2 Author affiliation: 1 College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang, China 2 National Research Center for Chinese Minority Medicine, Minzu University of China, Beijing 100081, China Corresponding author: Yin, X.-Y. (ncyxy@hotmail.com) Source title: Journal of Separation Science Abbreviated source title: J. Sep. Sci. Volume: 35 Issue: 3 Issue date: February 2012 Publication year: 2012 Pages: 384-391 Language: English ISSN: 16159306 E-ISSN: 16159314 CODEN: JSSCCJ Document type: Journal article (JA) Publisher: Wiley-VCH Verlag, P.O. Box 101161, Weinheim, D-69451, Germany Abstract: A novel method, micro-solid-phase extraction based on membrane-protected molecularly imprinted polymer, was developed to extract hyperoside and isoquercitrin in rat plasma. Synthesized hyperoside MIPs were packed in a porous polyether sulfone membrane envelope to perform extraction. The parameters sorbent materials, membrane types, extraction time and desorption conditions were optimized for micro-solid-phase extraction. Under the optimal conditions, correlation coefficients, 0.998 and 0.999, were obtained for hyperoside and isoquercitrin, respectively, with the linear range between 1 and 120μg/mL. The absolute extraction recoveries from 84.5 to 89.3% were found. The method detection limits of hyperoside and isoquercitrin were 0.24 and 0.22μg/mL, respectively. Compared with traditional methods, solid-phase extraction, liquid-liquid extraction and protein precipitation, the developed method was simple, highly efficient for extraction, environmentally friendly, and particularly suitable for complex biological samples. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Number of references: 20 Main heading: Solvent extraction Controlled terms: Body fluids - Desorption - Ethers - High performance liquid chromatography - Liquids - Optimization - Phase separation - Plasma waves - Rats Uncontrolled terms: Biological samples - Correlation coefficient - Environmentally-friendly - Extraction recovery - Extraction time - Kendir extract - Linear range - Liquid-liquid extraction - Membrane types - Method detection limits - Molecularly Imprinted Polymer - Optimal conditions - Polyether sulfone - Protein precipitation - Rat plasma - Solid-phase extraction - Sorbent materials Classification code: 932.3 Plasma Physics - 931.2 Physical Properties of Gases, Liquids and Solids - 921.5 Optimization Techniques - 804.1 Organic Compounds - 802.3 Chemical Operations - 461.9 Biology - 461.2 Biological Materials and Tissue Engineering DOI: 10.1002/jssc.201100867 Database: Compendex |
2樓2012-07-18 20:05:43
ncyxoy
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