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love36183618木蟲 (初入文壇)
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論文摘要部分，大家?guī)蛶兔�，謝謝啦
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| DNA是生物的基本遺傳物質(zhì),也是生命的物質(zhì)基礎(chǔ).定量分析、特異性識(shí)別DNA分子,對(duì)基因?qū)W、分析生物學(xué)、病毒學(xué)等相關(guān)學(xué)科的發(fā)展具有非常重要的意義.目前,許多方法如熒光光譜法、紫外光譜法、電化學(xué)方法等都被用于DNA的研究.但是由于DNA分子自身的吸光,多采用熒光探針法檢測DNA.熒光探針法與傳統(tǒng)的方法相比,具有檢測快速、操作簡單、重復(fù)性好及輻射小等優(yōu)點(diǎn).在DNA自動(dòng)測序、疾病診斷、臨床醫(yī)學(xué)等方面已經(jīng)得到廣泛的應(yīng)用[5].有關(guān)用熒光光譜法研究DNA已經(jīng)引起了人們的廣泛關(guān)注.由于靈敏度是影響熒光探針檢測的重要因素,因此找出更加靈敏的熒光探針已經(jīng)成為當(dāng)前研究的熱點(diǎn).用于核酸分析的熒光探針,目前有以下幾類:有吖啶、菲啶類、熒光素、羅丹明類、菁類和金屬配合物等.本文用自主發(fā)現(xiàn)的新型的BCDO熒光探針分子,采用熒光猝滅法,通過熒光光譜的測定研究了BCDO與DNA的相互作用,并對(duì)它們之間的作用方式進(jìn)行了探討。 |
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shuxinme111
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【答案】應(yīng)助回帖
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sltmac: 金幣+30, 翻譯EPI+1 2012-08-20 21:49:25
sltmac: 金幣+30, 翻譯EPI+1 2012-08-20 21:49:25
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DNA is not only the main hereditary substance of biological object, but also the material basis of life. Quantitative Analysis and specific recognition of DNA molecular play a significant role in the progress of related subjects like genetics, analytical biology and virology. Nowadays, many methods including Fluorescence Spectroscopy , ultraviolet spectroscopy, electrochemical technique have been applied in the DNA study. However, due to the self light-absorption of DNA molecular, Fluorescence Probe Method is used mostly to detect DNA. Compared with traditional methods, Fluorescence Probe Method is merit in fast detection, simple operation, good reproduction and little radiation, which has been widely used in automated DNA sequencing, disease diagnosis and clinical medicine. Therefore, it has attracted wide attention to study DNA by Fluorescence Spectroscopy method. Since sensitivity is an important factor to effect the detection by Fluorescence Probe, how to find out a more sensitive Fluorescence Probe becomes a research hotspot. Fluorescence Probes currently used for nucleic acid analysis can be classified to following kinds: acridines, phenanthridines, fluoreseeins, rhodamines, cyanines and metal complexes. In this paper, applied a newly self-found BCDO Fluorescence Probe molecular and fluorescence quenching method, the interaction and action mode between BCDO and DNA were studied and explored by detecting the and the fluorescence spectra. 供樓主參考～～～～ |
2樓2012-07-24 11:23:23
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